Molecular mechanism of verbascoside in promoting acetylcholine release of neurotransmitter.
10.19540/j.cnki.cjcmm.20240802.702
- Author:
Zhi-Hua ZHOU
1
;
Hai-Yan XING
2
;
Yan LIANG
1
;
Jie GAO
1
;
Yang LIU
1
;
Ting ZHANG
1
;
Li ZHU
1
;
Jia-Long QIAN
1
;
Chuan ZHOU
1
;
Gang LI
1
Author Information
1. Inner Mongolia Medical University Hohhot 010110, China.
2. Department of Pharmacy, People's Hospital of Inner Mongolia Autonomous Region Hohhot 010017, China.
- Publication Type:Journal Article
- Keywords:
Alzheimer′s disease;
acetylcholine;
calcium ion;
verbascoside;
vesicle protein
- MeSH:
Animals;
Rats;
Glucosides/administration & dosage*;
Acetylcholine/metabolism*;
Alzheimer Disease/genetics*;
PC12 Cells;
Phenols/chemistry*;
Neurotransmitter Agents/metabolism*;
Drugs, Chinese Herbal;
Calcium-Calmodulin-Dependent Protein Kinase Type 2/genetics*;
Humans;
Phosphorylation/drug effects*;
Calcium/metabolism*;
Polyphenols
- From:
China Journal of Chinese Materia Medica
2025;50(2):335-348
- CountryChina
- Language:Chinese
-
Abstract:
The molecular mechanism of verbascoside(OC1) in promoting acetylcholine(ACh) release in the pathogenesis of Alzheimer's disease(AD) was studied. Adrenal pheochromocytoma cells(PC12) of rats induced by β-amyloid protein(1-42)(Aβ_(1-42)) were used as AD models in vitro and were divided into control group, model group(Aβ_(1-42) 10 μmol·L~(-1)), OC1 treatment group(2 and 10 μg·mL~(-1)). The effect of OC1 on phosphorylated proteins in AD models was analyzed by whole protein phosphorylation quantitative omics, and the selectivity of OC1 for calcium channel subtypes was virtually screened in combination with computer-aided drug design. The fluorescence probe Fluo-3/AM was used to detect Ca~(2+) concentration in cells. Western blot analysis was performed to detect the effects of OC1 on the expression of phosphorylated calmodulin-dependent protein kinase Ⅱ(p-CaMKⅡ, Thr286) and synaptic vesicle-related proteins, and UPLC/Q Exactive MS was used to detect the effects of OC1 on ACh release in AD models. The effects of OC1 on acetylcholine esterase(AChE) activity in AD models were detected. The results showed that the differentially modified proteins in the model group and the OC1 treatment group were related to calcium channel activation at three levels: GO classification, KEGG pathway, and protein domain. The results of molecular docking revealed the dominant role of L-type calcium channels. Fluo-3/AM fluorescence intensity decreased under the presence of Ca~(2+) chelating agent ethylene glycol tetraacetic acid(EGTA), L-type calcium channel blocker verapamil, and N-type calcium channel blocker conotoxin, and the effect of verapamil was stronger than that of conotoxin. This confirmed that OC1 promoted extracellular Ca~(2+) influx mainly through its interaction with L-type calcium channel protein. In addition, proteomic analysis and Western blot results showed that the expression of p-CaMKⅡ and downstream vesicle-related proteins was up-regulated after OC1 treatment, indicating that OC1 acted on vesicle-related proteins by activating CaMKⅡ and participated in synaptic remodeling and transmitter release, thus affecting learning and memory. OC1 also decreased the activity of AChE and prolonged the action time of ACh in synaptic gaps.
