Serum pharmacochemistry of Panacis Japonici Rhizoma extract based on UPLC-Q-Exactive Orbitrap-MS.
10.19540/j.cnki.cjcmm.20240910.203
- Author:
Yuan WEN
1
;
Chang LIU
1
;
Qi YUAN
1
;
Rui WANG
2
;
Xue-Qing LI
1
;
Ding YUAN
1
;
Cheng-Fu YUAN
2
;
Yu-Min HE
1
Author Information
1. Thirdgrade Pharmacological Laboratory on Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, China Three Gorges University Yichang 443002, China College of Medicine and Health Sciences, China Three Gorges University Yichang 443002, China.
2. Thirdgrade Pharmacological Laboratory on Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, China Three Gorges University Yichang 443002, China College of Basic Medical Sciences, China Three Gorges University Yichang 443002, China.
- Publication Type:Journal Article
- Keywords:
Panacis Japonici Rhizoma extract;
UPLC-Q-Exactive Orbitrap-MS;
metabolites;
prototype components;
serum pharmacochemistry
- MeSH:
Animals;
Rats;
Drugs, Chinese Herbal/administration & dosage*;
Chromatography, High Pressure Liquid/methods*;
Male;
Rats, Sprague-Dawley;
Rhizome/chemistry*;
Mass Spectrometry/methods*
- From:
China Journal of Chinese Materia Medica
2024;49(23):6490-6499
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the chemical components of Panacis Japonici Rhizoma extract and absorbed components in rats were identified by ultra-high performance liquid chromatography-quadrupole exactive orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS). The separation was performed by gradient elution on Waters UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) with the mobile phase of water and acetonitrile containing 0.1% formic acid. High resolution multistage mass spectrometry data were collected by electrospray ionization in positive and negative ion modes. The chemical components of Panacis Japonici Rhizoma extract were identified by comparing with the retention time, high resolution precise molecular weight, and secondary fragment ions of reference substances and related literature. After intragastric administration of Panacis Japonici Rhizoma extract, blood was collected from the abdominal aorta of rats for separation of the serum, and the absorbed components were scanned and identified. The results showed that 43 chemical components were detected in the Panacis Japonici Rhizoma extract, including 22 saponins, 9 amino acids, 5 polysaccharides, 2 volatile oils, and 5 nucleosides. In the serum, 18 components were detected, including 10 prototype components, 6 metabolites, and 2 unknown components. This study analyzed the chemical components and absorbed components of Panacis Japonici Rhizoma extract, providing clues for clarifying the pharmacological basis of Panacis Japonici Rhizoma.
