Effects of Xihuang Pills on angiogenesis, invasion, and metastasis of p rostate cancer based on FAK/Src/ERK pathway.
10.19540/j.cnki.cjcmm.20240909.401
- Author:
Yan LONG
1
;
Xin-Jun LUO
2
;
Bo ZOU
1
;
Xin-Jun DAI
3
;
Fang-Zhi FU
1
;
Biao WANG
1
;
Li-Tong WU
1
;
Yong-Rong WU
4
;
Qing ZHOU
2
;
Xue-Fei TIAN
5
Author Information
1. Department of Andrology, the First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007, China Hunan Provincial Key Laboratory of Traditional Chinese Medicine Prescription and Transformation, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Tumor Prevention Mechanism of Traditional Chinese Medicine,Hunan University of Chinese Medicine Changsha 410208, China.
2. Department of Andrology, the First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007, China.
3. Hunan Provincial Key Laboratory of Traditional Chinese Medicine Prescription and Transformation, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Tumor Prevention Mechanism of Traditional Chinese Medicine,Hunan University of Chinese Medicine Changsha 410208, China Liuyang Hospital of Traditional Chinese Medicine Affiliated to Hunan University of Chinese Medicine Changsha 410300, China.
4. Hunan Provincial Key Laboratory of Traditional Chinese Medicine Prescription and Transformation, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Tumor Prevention Mechanism of Traditional Chinese Medicine,Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Traditional Chinese Medicine Tumour in Hunan Universities, Hunan University of Chinese Medicine Changsha 410208, China College of Traditional Chinese Medicine, Hunan University of Chinese Medicine Changsha 410208, China.
5. Hunan Provincial Key Laboratory of Traditional Chinese Medicine Prescription and Transformation, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Tumor Prevention Mechanism of Traditional Chinese Medicine,Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Traditional Chinese Medicine Tumour in Hunan Universities, Hunan University of Chinese Medicine Changsha 410208, China College of Integrative Medicine, Hunan University of Chinese Medicine Changsha 410208, China.
- Publication Type:Journal Article
- Keywords:
FAK/Src/ERK signalling pathway;
Xihuang Pills;
angiogenesis;
prostate cancer
- MeSH:
Humans;
Male;
Prostatic Neoplasms/enzymology*;
Drugs, Chinese Herbal/chemistry*;
Animals;
Mice;
Cell Proliferation/drug effects*;
Mice, Nude;
Cell Movement/drug effects*;
Cell Line, Tumor;
src-Family Kinases/genetics*;
Neovascularization, Pathologic/metabolism*;
Neoplasm Metastasis;
Neoplasm Invasiveness;
Focal Adhesion Kinase 1/genetics*;
Extracellular Signal-Regulated MAP Kinases/genetics*;
MAP Kinase Signaling System/drug effects*;
Focal Adhesion Protein-Tyrosine Kinases/genetics*;
Signal Transduction/drug effects*;
Angiogenesis
- From:
China Journal of Chinese Materia Medica
2024;49(23):6378-6388
- CountryChina
- Language:Chinese
-
Abstract:
Based on the focal adhesion kinase(FAK)/steroid receptor coactivator(Src)/extracellular regulated protein kinase(ERK) pathway, this study explored the effects of Xihuang Pills on angiogenesis, invasion, and metastasis in prostate cancer. Liquid chromatography-tandem mass spectrometry(LC-MS/MS) was used to analyze and identify the active ingredients of Xihuang Pills. Bioinformatics techniques, including R language and Perl programs, were employed to analyze the interactions between prostate cancer-related targets and the potential targets of Xihuang Pills. A subcutaneous transplantation tumor model of prostate cancer was established in nude mice using PC3 cells to verify the efficacy and molecular mechanisms of Xihuang Pills. In vitro cellular experiments, including cell proliferation assays(CCK-8), Transwell assays, scratch assays, real-time quantitative reverse transcription PCR, and Western blot, were used to detect the effects of Xihuang Pills on the proliferation, invasion, and migration of prostate cancer cells, as well as on FAK/Src/ERK pathway-related targets. LC-MS/MS identified 99 active ingredients in Xihuang Pills, including gallic acid, gentisic acid, artemisinin, corilagin, phenylbutazone-glucoside, thujic acid, and arecoic acid B. Network pharmacological analysis of the active ingredients in Xihuang Pills revealed that the FAK/Src/ERK signaling pathway was a key pathway in its anti-prostate cancer effects. In vivo and in vitro experiments confirmed that Xihuang Pills significantly inhibited the proliferation, invasion, and migration of PC3 and LNCaP cells, suppressed the growth of PC3 subcutaneous tumors, and reduced the protein expression levels related to the FAK/Src/ERK signaling pathway. In conclusion, the inhibition of angiogenesis, invasion, and metastasis by regulating the FAK/Src/ERK pathway is one of the mechanisms by which Xihuang Pills exert anti-prostate cancer effects.
