Establishment of a standard DSS library for identification of original plants of medicinal materials in Chinese Pharmacopoeia.
10.19540/j.cnki.cjcmm.20240827.102
- Author:
Duo-Mei WANG
1
;
Chao JIANG
2
;
Jing-Zhe PU
1
;
Chong HU
1
;
Ling-Li CHEN
1
;
Ya-Zhong ZHANG
1
;
Yuan YUAN
3
Author Information
1. Institute for Drug Control, Anhui Institute of Food and Drug Control Hefei 230051, China NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine Hefei 230051, China.
2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Experimental Research Center,China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
DNA signature sequence;
identification of original plants;
standard nucleotide sequence
- MeSH:
Plants, Medicinal/classification*;
DNA, Plant/genetics*;
Drugs, Chinese Herbal;
Pharmacopoeias as Topic;
Genetic Markers;
Medicine, Chinese Traditional
- From:
China Journal of Chinese Materia Medica
2024;49(23):6249-6256
- CountryChina
- Language:Chinese
-
Abstract:
With the development of molecular pharmacognosy, the advantages of DNA molecular markers in the identification of original plants of Chinese medicinal materials are becoming increasingly significant. To compensate for the limitations of existing markers in the quality supervision of Chinese medicinal materials, our team has independently designed a new molecular marker named DNA signature sequence(DSS). This marker is a nucleotide sequence that only appears in a specific taxonomic unit, with a length of 40 bp and high identification accuracy. This article aims to screen and verify the DSS markers that can accurately identify the original plants of the medicinal materials included in the volume one of the Chinese Pharmacopoeia, establish the operating procedure for developing standard nucleotide sequences, and lay a foundation for the widespread application of polymerase chain reaction in the quality supervision of traditional Chinese medicine. Firstly, the Chloroplast Genome Information Resource(CGIR) was searched for the chloroplast genome sequences of the test samples, species of the same genus, and common background species. IdenDSS was used to obtain the DSS tags and specific identification primers of the tested species. After DNA extraction, PCR amplification, sequencing, and sequence alignments, a total of 203 DSS markers of Chinese medicinal materials were obtained for validation. The above sequences were uploaded to the Traditional Chinese Medicine Molecular Identification Platform(www.herbsdna.com), and a standard DSS library was established for identifying the original plants of medicinal materials, serving as an important tool for quality supervision of Chinese materia medica. On this basis, an operating procedure for DSS development is formed, laying a foundation for further DSS screening and application based on more diverse genome sequences.
