Chemical constituents and anti-inflammatory activity of Dracocephalum tanguticum.
10.19540/j.cnki.cjcmm.20240713.201
- Author:
Ting-Ting WANG
1
;
Zhao-Yue DONG
2
;
Yong-Sheng WEI
3
;
Hong QUAN
1
;
Xiao-Zhong LAN
1
Author Information
1. Key Laboratory of Tibetan Medicine Resources Conservation and Utilization of Tibet Autonomous Region, Xizang Agricultural and Animal Husbandry University Nyingchi 860000, China the Provincial and Ministerial Co-founded Collaborative Innovation Center for R&D in Xizang Characteristic Agricultural and Animal Husbandry Resources, Xizang Agricultural and Animal Husbandry University Nyingchi 860000, China the Center for Xizang Chinese (Tibetan) Medicine Resource,Xizang Agricultural and Animal Husbandry University Nyingchi 860000, China Joint Laboratory for Tibetan Materia Medica Resources Scientific Protection and Utilization Research of Tibetan Medical Research Center of Xizang Nyingchi 860000, China.
2. Chongqing Key Laboratory of New Drug Screening from Traditional Chinese Medicine, College of Pharmaceutical Sciences,Southwest University Chongqing 400715, China.
3. Key Laboratory of Tibetan Medicine Resources Conservation and Utilization of Tibet Autonomous Region, Xizang Agricultural and Animal Husbandry University Nyingchi 860000, China the Provincial and Ministerial Co-founded Collaborative Innovation Center for R&D in Xizang Characteristic Agricultural and Animal Husbandry Resources, Xizang Agricultural and Animal Husbandry University Nyingchi 860000, China the Center for Xizang Chinese (Tibetan) Medicine Resource,Xizang Agricultural and Animal Husbandry University Nyingchi 860000, China Joint Laboratory for Tibetan Materia Medica Resources Scientific Protection and Utilization Research of Tibetan Medical Research Center of Xizang Nyingchi 860000, China Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing Academy of Chinese Materia Medica Chongqing 400065, China.
- Publication Type:Journal Article
- Keywords:
Dracocephalum tanguticum;
anti-inflammatory activity;
chemical constituents;
dracotangusion C
- MeSH:
Animals;
Mice;
Anti-Inflammatory Agents/chemistry*;
RAW 264.7 Cells;
Drugs, Chinese Herbal/pharmacology*;
Lamiaceae/chemistry*;
Nitric Oxide;
Macrophages/immunology*;
Molecular Structure
- From:
China Journal of Chinese Materia Medica
2024;49(22):6173-6180
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of Dracocephalum tanguticum were investigated using normal-and reverse-phase silica gel column chromatography, RP-HPLC, and other separation techniques, combined with experimental methods such as NMR, UV, IR, MS, and ORD, as well as comparison with reported literature data. From the ethanol extract of D. tanguticum, 10 compounds were isolated and identified: dracotangusion C(1),(1R,4S,5S,10R)-(+)-germacrone-1(10)-4-diepoxide(2), 1β,4β,5β-trihydroxy-7(11),9-germacradien-8-one(3), curcumadione(4), β-sitosterol(5), lilacoside(6), diosmetin-7-O-β-D-glucopyranoside(7), diosmin(8), luteolin-7-O-glucoside(9), and luteolin(10). Among these, compound 1 is a novel sesquiterpenoid, while compounds 2-4 represent the first isolation of sesquiterpenoid components from this genus. By measuring the release of NO in the culture medium of lipopolysaccharide(LPS)-induced RAW264.7 macrophages, it was found that at an administration concentration of 20 μmol·L~(-1), all 10 compounds significantly inhibited the LPS-induced release of NO(P < 0.01), exhibiting anti-inflammatory effects.
