Isolation and identification of steroids from Cyanotis arachnoidea and their anti-HSV-1 activities in vitro.
10.19540/j.cnki.cjcmm.20240808.202
- Author:
Fang-Yi SU
1
;
Bo LI
2
;
Bin XIAO
2
;
Hong WAN
2
;
Ding LIU
2
;
Chun-Lei ZHANG
3
Author Information
1. School of Life Science, Zhengzhou Normal University Zhengzhou 450044, China.
2. Chengdu Push Bio-Technology Co., Ltd. Chengdu 610045, China.
3. School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing 211198, China.
- Publication Type:Journal Article
- Keywords:
Cyanotis arachnoidea;
HSV-1;
chemical constituent;
structural identification
- MeSH:
Herpesvirus 1, Human/drug effects*;
Antiviral Agents/isolation & purification*;
Animals;
Chlorocebus aethiops;
Steroids/isolation & purification*;
Vero Cells;
Drugs, Chinese Herbal/isolation & purification*;
Molecular Structure
- From:
China Journal of Chinese Materia Medica
2024;49(22):6165-6172
- CountryChina
- Language:Chinese
-
Abstract:
The steroid constituents in the 75% ethanol extract of Cyanotis arachnoidea and their anti-HSV-1 activities in vitro were investigated in this study. The ethyl acetate fraction of the extract was isolated and purified using silica gel, ODS, Sephadex LH-20 column chromatography, and semi-preparative HPLC. Structural identification was conducted based on spectroscopic data. Ten steroid compounds were isolated and identified, namely makisterone A 3-acetate(1), makisterone A 2-acetate(2), makisterone A(3), ajugasterone C 2-acetate(4), ajugasterone C(5), 20-hydroxyecdysone 2-acetate(6), 20-hydroxyecdysone 3-acetate(7), podecdysone C(8), rubrosterone(9), and poststerone(10). Compounds 1 and 2 are new steroid compounds. The anti-HSV-1 activities of compounds 1-10 were evaluated using the CPE inhibition method. Vero cells were used in the experiment, with acyclovir as the positive control. The results showed that compounds 9 and 10 showed anti-HSV-1 activity, with EC_(50) values of 83.11 and 103.62 μg·mL~(-1), respectively.
