Analysis of chemical constituents of different processed products of Strychni Semen based on UPLC-Q-TOF-MS technique.
10.19540/j.cnki.cjcmm.20240809.301
- Author:
Xian ZHANG
1
;
Hai-Lian ZHENG
1
;
Na ZHANG
1
;
Cheng XU
1
;
Mei-Qi LU
1
;
Ling-Bang MENG
1
;
Huan YU
1
Author Information
1. School of Pharmacy, Jiangxi University of Chinese Medicine Nanchang 330004, China.
- Publication Type:English Abstract
- Keywords:
Strychni Semen;
chemical composition;
multivariate statistical analysis;
processing;
ultraperformance liquid chromatography-quadrupole-time of flight tandem mass spectrometry(UPLC-Q-TOF-MS)
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Drugs, Chinese Herbal/analysis*;
Mass Spectrometry/methods*;
Strychnos/chemistry*;
Principal Component Analysis
- From:
China Journal of Chinese Materia Medica
2024;49(22):6138-6148
- CountryChina
- Language:Chinese
-
Abstract:
This study utilized ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) to rapidly analyze and identify the chemical constituents in five processed products of Strychni Semen(raw, sand-roasted, fried, urine-soaked, and vinegar-processed products). Using PeakView software to extract compound information, 50 chemical components were identified based on retention time, accurate molecular ion peaks, secondary mass spectrometry data, and comparison with reference standards and relevant literature. Specifically, 41 components were identified in raw Strychni Semen, 48 in sand-roasted, 43 in fried, 41 in urine-soaked, and 40 in vinegar-processed products. Principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) were employed to identify differential components among the processed products, with variable importance in projection(VIP) values>1 and a t-test with P<0.05 as criteria. PCA revealed significant differences among the five processed products, demonstrating good clustering and separation. OPLS-DA identified 13 differential components, including brucine, strychnine, loganic acid, and chlorogenic acid. The results indicated that S. nux-vomica primarily contained alkaloids, organic acids, and glycosides, with significant compositional differences among the various processed products. Notably, the content of key components such as brucine and strychnine decreased after processing, with the most significant reduction observed in vinegar-processed Strychni Semen. In conclusion, this study provides a comprehensive comparison of the effects of different processing methods on the chemical composition of Strychni Semen, exploring the differences in the material basis of the processed products and offering data to support the scientific rationale behind the processing of Strychni Semen.
