Genomic information mining reveals Rehmannia glutinosa growth-promoting mechanism of endophytic bacterium Kocuria rosea.
10.19540/j.cnki.cjcmm.20240814.102
- Author:
Lin-Lin WANG
1
;
Gui-Xiao LA
2
;
Xiu-Hong SU
3
;
Lin-Lin YANG
1
;
Lei-Xia CHU
1
;
Jun-Qi GUO
4
;
Cong-Long LIAN
1
;
Bao ZHANG
1
;
Cheng-Ming DONG
3
;
Sui-Qing CHEN
3
;
Chun-Yan WANG
1
Author Information
1. School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
2. Institute of Chinese Herbal Medicines, Henan Academy of Agricultural Sciences Zhengzhou 450002, China.
3. School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan &Education Ministry of P.R.China Zhengzhou 450046, China.
4. Qinyang Agricultural Technology Promotion Center Qinyang 454550, China.
- Publication Type:Journal Article
- Keywords:
Kocuria rosea;
Rehmannia glutinosa;
genome sequencing;
growth-promoting effect;
interaction mechanism;
nutrient elements
- MeSH:
Rehmannia/metabolism*;
Endophytes/metabolism*;
Plant Roots/growth & development*;
Micrococcaceae/genetics*;
Data Mining;
Plant Leaves/metabolism*;
Genomics;
Rhizosphere
- From:
China Journal of Chinese Materia Medica
2024;49(22):6119-6128
- CountryChina
- Language:Chinese
-
Abstract:
This study explored the growth-promoting effect and mechanism of the endophytic bacterium Kocuria rosea on Rehmannia glutinosa, aiming to provide a scientific basis for the development of green bacterial fertilizer. R. glutinosa 'Jinjiu' was treated with K. rosea, and the shoot parameters including leaf length, leaf width, plant width, and stem diameter were measured every 15 days. After 120 days, the shoots and roots were harvested. The root indicators(root number, root length, root diameter, root fresh weight, root dry weight, root volume, and root vitality) and secondary metabolites(catalpol, rehmannioside A, rehmannioside D, verbascoside, and leonuride) were determined. The R. glutinosa growth-promoting mechanism of K. rosea was discussed from the effect of K. rosea on the nutrient element content in R. glutinosa and rhizosphere soil and the genome information of this plant. After application of K. rosea, the maximum increases in leaf length, leaf width, plant width, and stem diameter were 35.67%(60 d), 25.39%(45 d), 40.17%(60 d), and 113.85%(45 d), respectively. The root number, root length, root diameter, root volume, root fresh weight, root dry weight, and root viability increased by 41.71%, 45.10%, 48.61%, 94.34%, 101.55%, 147.61%, and 42.08%, respectively. In addition, the content of rehmannioside A and verbascoside in the root of R. glutinosa increased by 76.67% and 69.54%, respectively. K. rosea promoted the transformation of nitrogen(N), phosphorus(P), and potassium(K) in the rhizosphere soil into the available state. Compared with that in the control, the content of available N(54.60 mg·kg~(-1)), available P(1.83 μmol·g~(-1)), and available K(83.75 mg·kg~(-1)) in the treatment with K. rosea increased by 138.78%, 44.89%, and 14.34%, respectively. The content of N, P, and K in the treatment group increased by 293.22%, 202.63%, and 23.80% in the roots and by 23.60%, 107.23%, and 134.53% in the leaves of R. glutinosa, respectively. K. rosea carried the genes related to colonization(rbsB, efp, bcsA, and gmhC), N, P, and K metabolism(narG, narH, narI, nasA, nasB, GDH2, pyk, aceB, ackA, CS, ppa, ppk, ppk2, pstS, pstA, pstB, and pstC), and indole-3-acetic acid and zeatin synthesis(iaaH and miaA). Further studies showed that K. rosea could colonize the roots of R. glutinosa and secrete indole-3-acetic acid(3.85 μg·mL~(-1)) and zeatin(0.10 μg·mL~(-1)). In summary, K. rosea promotes the growth of R.ehmannia glutinosa by enhancing the nutrient uptake, which provides a theoretical basis for the development of plant growth-promoting microbial products.
