Identification and expression profiling of Dof transcription factor family in Aesculus chinensis.
10.19540/j.cnki.cjcmm.20240813.101
- Author:
Chen QIN
1
;
Xue-Hua XIE
2
;
Lu LI
3
;
Jun LI
2
;
Yao-Lei MI
2
;
Hui-Yan CAO
1
;
Wei SUN
2
;
Wei MA
1
;
Hui-Hua WAN
2
;
Xiu-Bo LIU
4
Author Information
1. School of Pharmacy, Heilongjiang University of Chinese Medicine Harbin 150040, China.
2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
3. School of Pharmacy, Guangdong Pharmaceutical University Guangzhou 510006, China.
4. School of Pharmacy, Heilongjiang University of Chinese Medicine Harbin 150040, China Jiamusi College, Heilongjiang University of Chinese Medicine Jiamusi 154007, China.
- Publication Type:Journal Article
- Keywords:
Aesculus chinensis;
Dof transcription factor;
biosynthesis regulation;
gene family identification
- MeSH:
Plant Proteins/metabolism*;
Phylogeny;
Gene Expression Regulation, Plant;
Transcription Factors/metabolism*;
Gene Expression Profiling;
Multigene Family
- From:
China Journal of Chinese Materia Medica
2024;49(22):6082-6092
- CountryChina
- Language:Chinese
-
Abstract:
Aesculus chinensis is an important medicinal and horticultural plant. Its dried mature seeds, known as "Suoluozi", are a well-known traditional Chinese medicine. Aescins are its main active components, possessing multiple pharmacological activities such as anti-inflammatory and anti-exudative effects. They are widely used in the treatment of diseases such as lumbar disc herniation, postoperative edema, and sports injuries, leading to a continuous increase in market demand in recent years. The DNA binding with one finger(Dof) family is a unique transcription factor family found in the plant kingdom. It plays a crucial role in plant growth, development, stress responses, and regulation of secondary metabolism. However, research on the Dof gene family in A. chinensis is relatively scarce. In this study, we identified 36 AcDof genes from the genome of A. chinensis and analyzed their physicochemical properties, chromosomal localization, phylogenetic relationships, gene structures, cis-acting elements, and expression patterns in different tissues. The results showed that AcDof proteins ranged from 81 to 493 amino acids in length, with molecular weights ranging from 9 270.38 to 55 015.68 and isoelectric points ranging from 4.84 to 10.2. The subcellular localization analysis revealed that 34 AcDof proteins were located in the nucleus, while the remaining two AcDof proteins were located in the chloroplasts. Phylogenetic analysis divided AcDofs into nine subgroups, and gene structure analysis indicated that all AcDof genes possessed a C2-C2 type single zinc finger domain. Gene expression analysis using transcriptome data revealed tissue-specific expression patterns among AcDof family members. Specifically, AcDof04, AcDof02, and AcDof03 exhibited specific expression in seeds, suggesting their potential involvement in the regulation of aescin biosynthesis. This study not only enhances our understanding of the Dof gene family in A. chinensis but also provides important genetic resources for further investigation of the functions and regulatory mechanisms of Dof genes in this species.
