Characterization of the shared microbial profile between infected extraction socket and maxillary sinus in patients with odontogenic maxillary sinusitis
10.12016/j.issn.2096-1456.202550322
- VernacularTitle:牙源性上颌窦炎患者拔牙窝与上颌窦共有微生物谱的特征分析
- Author:
LU Chang
1
;
QIN Yicheng
2
;
WANG Ye
1
;
XU Min
1
;
LIN Jiang
1
Author Information
1. Beijing Tongren Hospital, Capital Medical University
2. Hangzhou Stomatology Hospital
- Publication Type:Journal Article
- Keywords:
odontogenic maxillary sinusitis;
microbial migration;
high-throughput sequencing;
Fusobacterium nucleatum;
Porphyromonas;
endodontic pathogens;
periodontal pathogens;
16S rRNA
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2025;33(12):1041-1052
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether infected granulation tissue in tooth extraction sockets and maxillary sinus pus share a common microbial profile at the subspecies-strain level in patients with odontogenic maxillary sinusitis (OMS), providing evidence for infection origin tracing and precise antimicrobial therapy in OMS.
Methods:This study was reviewed and approved by the institutional ethics committee. Nine consecutive OMS patients who underwent synchronous endoscopic sinus surgery and tooth extraction from October 2020 to August 2022 were prospectively enrolled. Under general anesthesia, paired specimens were collected from infected extraction-socket granulation tissue and maxillary sinus pus. Bacterial DNA was extracted, and the full-length 16S rRNA gene was sequenced on the Illumina MiSeq platform. Amplicon sequence variants (ASVs) were generated using the DADA2 algorithm and taxonomically annotated to the subspecies level against the Human Oral Microbiome Database. The detection rate of shared ASVs between the two sites and their relative abundance in sinus pus were compared. Functional profiles were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUSt2).
Results: Shared ASVs were identified in seven of the nine patients. Fusobacterium, Parvimonas, Porphyromonas, and Prevotella were the most prevalent genera. Porphyromonas gingivalis and Fusobacterium nucleatum were co-detected in multiple patients, with relative abundances exceeding 5% in sinus pus of several cases. Identical ASVs of F. nucleatum or Porphyromonas spp. were detected in six patients; the ASVs corresponding to F. nucleatum subsp. nucleatum and Porphyromonas endodontalis were significantly more abundant in sinus pus than in extraction-socket granulation tissue. PICRUSt2 functional profiling revealed that the proportion of socket-derived microbes in sinus pus was strongly correlated with 10 pathways, including ferroptosis, adipocytokine signaling, and apoptosis, et al. Except for biotin metabolism, the remaining pathways showed weak correlation with the proportion of extraction socket-derived ASVs in the extraction-socket granulation tissue and maxillary sinus pus. Removing F. nucleatum ASVs markedly attenuated these associations
Conclusion:At the subspecies-strain level, this study confirmed the presence of a shared microbial profile between infected extraction-socket granulation tissue and maxillary sinus pus in patients with odontogenic maxillary sinusitis. The co-detected subspecies-strains with high relative abundance in maxillary sinus pus included Fusobacterium nucleatum subsp. nucleatum and Porphyromonas endodontalis, thus providing strain-level microbiological evidence for infection source tracing in OMS.
- Full text:2025121511221990326牙源性上颌窦炎患者拔牙窝与上颌窦共有微生物谱的特征分析.pdf
