Results of viral and bacterial pathogens detected in children hospitalized with pneumonia
- VernacularTitle:Уушигны хатгаа оношоор хэвтэн эмчлүүлж буй хүүхдэд вирус болон бактерийн халдварыг илрүүлсэн дүн
- Author:
Uugantsetseg E
1
;
Miyesuren E
2
;
Orlom D
2
;
Tsevegmid U
2
;
Oyungerel R
2
;
Khosbayar T
2
Author Information
1. Department of Clinical Laboratory, School of Medicine, MNUMS
2. Clinical Molecular Diagnostic Center, MNUMS
- Publication Type:Journal Article
- Keywords:
Multiplex RT-PCR, Influenza, Influenza-like illness, SARS-CoV-2, Pulmonary consolidation, Pediatric respiratory disease, Co-infection
- From:
Mongolian Journal of Health Sciences
2025;89(5):133-137
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Respiratory viral and bacterial infections are one of the leading causes of hospitalization and death worldwide,
particularly affecting children, the elderly, and immune compromised individuals. The detection of causative pathogens
is crucial for understanding the epidemiology, prevention, management, and treatment of severe pneumonia. Molecular
diagnostic methods provide high sensitivity and speed, enabling the simultaneous detection of viral and bacterial
co-infections.
Aim:Detection of single and combined viral and bacterial infections in hospitalized children with pneumonia using
multiplex PCR.
Materials and Methods:We conducted a cross-sectional study design involving 100 hospitalized participants at the National
Center for Mothers, Newborns, and Women II. The study utilized questionnaires and laboratory analysis methods.
Nasopharyngeal swabs were collected from the participants using a sterile swab and transported in a UTM transport medium
(Jiangsu, China). After nucleic acid extraction using the STARMag 96 ProPrepC assay, the samples were analyzed
with the Seeprep 32 automated analyzer (Seegene Inc., Korea). Real-time polymerase chain reaction (PCR) analysis was
performed using the Allplex™ RV Master Assay and Allplex™ PneumoBacter Assay (Seegene Inc., Korea), with the
CFX-96 system (BioRad Inc., USA).
Results:The prevalence of viral infections in the study population was as follows: influenza type A 3% (n=3), influenza
type B 3% (n=3), total influenza virus 14% (n=14), respiratory syncytial virus 18% (n=18), adenovirus 10% (n=10),
rhinovirus 20% (n=20), and SARS-CoV-2 5% (n=5). No bacterial infections, such as B.parapertussis or L.pneumophila,
were detected. However, bacterial infections identified included B.pertussis in 1% (n=1), C.pneumoniae in 2% (n=2),
H.influenzae in 43% (n=43), M.pneumoniae in 52% (n=52), and S.pneumoniae in 45% (n=45). Of the 100 children in the
study, 6% had influenza or influenza-like illness. Among these, 43% had the virus alone, and 14% had a viral co-infection.
Bacterial infections were detected individually in 40% of cases, with co-infections in 46% of bacterial cases.
Conclusion:Metapneumovirus, B.parapertussis, and Legionella pneumophila were not detected in the study participants
(0%). Among the viral infections, respiratory syncytial virus (18%, n=18) and rhinovirus (20%, n=20) had the highest
prevalence. For bacterial infections, H.influenzae (43%, n=43), M.pneumoniae (52%, n=52), and S.pneumoniae (45%,
n=45) were most commonly detected.
- Full text:2025121011381863529Уушигны хатгаа оношоор хэвтэн эмчлүүлж буй хүүхдэд вирус болон бактерийн халдварыг.pdf
