Effects of Short-term High-salt Diet on Airway Inflammation in Mice with Allergic Asthma
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0622
- VernacularTitle:短期高盐饮食对过敏性哮喘小鼠气道炎症的影响
- Author:
Xing-yue GUO
1
;
Qin LI
2
;
Yun-qin SHEN
2
;
Ze-zhong TIAN
2
;
Jin-chao ZOU
3
;
Yan YANG
2
;
Yan-qiu CHEN
4
Author Information
1. School of Public Health， Sun Yat-sen University， Guangzhou 510080， China
2. Guangdong Provincial Key Laboratory of Food， Nutrition and Health， School of Public Health， Sun Yat-sen University（Guangzhou Campus）， Guangzhou 510080， China
3. Department of Medical Record Statistics， Sun Yat-sen University Cancer Center， Guangzhou 510060， China
4. Department of Otolaryngology， Guangzhou Women and Children Medical Centre， Guangzhou 510623， China
- Publication Type:Journal Article
- Keywords:
allergic asthma;
high salt diet;
airway inflammation;
eosinophils;
T cells
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(6):864-873
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of high-salt diet on airway inflammation in mice with allergic asthma. MethodsTwenty 4-week-old female BALB/c mice were randomly divided into four groups， including control group， allergic asthma model group， 4% high-salt diet group and 8% high-salt diet group. Ovalbumin（OVA）was injected intraperitoneally and inhaled to make an asthmatic model. The control group and the model group were fed ordinary diet and drinking water， and the high-salt groups were fed 4% or 8% high-salt diet and 1% saline respectively. After 25 days， the nasal symptoms of each group of mice were observed at the last challenge. The mice were sacrificed 24 hours later. The inflammatory cells of different subtype in the blood were measured by five classification of blood cell analysis. Hematoxylin-eosin （HE） staining was used to observe the infiltration of eosinophils， and periodic acid-schiff （PAS） staining was used to observe the goblet cell proliferation in the lung tissue. Flow cytometry was used to detect the proportion of helper T cells 1（Th1 cells）， Th2， Th17 cells and regulatory T cells （Treg cells） in the lung tissue， and the enzyme-linked immunosorbent assay was used to determine the levels of cytokines interleukin-4 （IL-4）， IL-5， IL-13， and IL-17 of bronchoalveolar lavage fluid （BALF）. ResultsCompared with the allergic asthma model group， 4% and 8% high-salt diet significantly reduced the proportion of eosinophils in blood， the proportion of Th17 cells in lung tissue， and IL-13 and IL-17 levels in BALF， but he effect of 8% high-salt diet on IL-13 was more significant than that of 4% high-salt diet （P<0.05）. However， only 8% high-salt diet significantly relieved nasal symptom， improved eosinophil infiltration and goblet cell proliferation， reduced the proportion of Th2 cells and Th2/ Th1 ratio in lung tissue and the levels of IL-4 and IL-5 in BALF （P<0.05）. ConclusionsShort-term high-salt diet can alleviate airway inflammation in mice with allergic asthma， and the effects of 8% high-salt diet are better than that of 4% high-salt diet.
