Molecular identification of bacteria from osteomyelitis of the jaws.
- Author:
Mi Sung KIM
1
;
Su Gwan KIM
;
Hae Man CHUNG
;
Sang Gon KIM
;
Joong Ki KOOK
;
Mi Kwang KIM
;
Hwa Sook KIM
;
So Young YOO
Author Information
1. Department of Oral &Maxillofacial Surgery, College of Dentistry, Chosun University, Korea. SGCKIM@mail.chosun.ac.kr
- Publication Type:Original Article
- MeSH:
Bacteria*;
Clone Cells;
Cloning, Organism;
Genes, rRNA;
Humans;
Jaw*;
Needles;
Osteomyelitis*;
Suppuration
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2003;29(1):48-55
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to isolate and identify the bacteria in osteomyelitis lesion of 3 patients. Two lesions were due to the postinfection after extraction. The other was resulted from mal-fixation of both sides of mandibular angles. Pus samples were collected by needle aspiration from the lesion and examined by culture method. Bacterial culture was performed in three culture systems (anaer-obic, CO2, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene cloning and nucleotide sequencing method. Our results showed that Streptococci species was predominantly isolated in both lesions of extraction socket. Only one species (Proteus vulagris) was detected in lesion of mandibular angle. This study was not sufficient to identify the causative bacteria in those osteomyelitis. However, our data may be offered the clue to solve the problem.
