Analysis of 68 samples with HIV-2 specific bands in western blot tests
10.3969/j.issn.1006-2483.2025.06.034
- VernacularTitle:68份免疫印迹试验出现HIV-2特异性条带的样本分析
- Author:
Dan ZHU
1
;
Yanlin ZHANG
1
;
Shanshan LI
1
;
Ling DU
1
Author Information
1. STD and AIDS testing Department, Chengdu Center for Disease Control and Prevention, Chengdu, Sichuan 610041, China
- Publication Type:Journal Article
- Keywords:
HIV-2;
Western blot (WB);
Line immunoassay (LIA);
Nucleic acid test;
Gene sequencing
- From:
Journal of Public Health and Preventive Medicine
2025;36(6):152-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the causes of HIV-2 specific bands in the Western blot (WB) tests and to understand previous HIV-2 infection status in this city. Methods A total of 68 samples with HIV-2 specific bands in WB were analyzed using two confirmatory reagents. The test results were further analyzed in combination with epidemiological data, nucleic acid testing and gene sequencing. Results When tested with MP reagent, 66 samples (97.06%) were found to be positive for HIV-2 antibody, while the other two were negative or undetermined for HIV-2 antibody. When tested with MIKROGEN reagent, 67 samples (98.53%) were found to be positive for HIV-1 antibody, and one sample was negative for HIV-1 antibody. Further HIV-1 nucleic acid testing was conducted on these samples, and all 68 samples tested positive for HIV-1 RNA, with the results all exceeding 5,000 copies/ml. After BLAST comparison, it was found that the homology similarity of 68 samples to the HIV-1 reference strain sequence was >90%, but there was no similarity with the HIV-2 reference strain sequence. Conclusion The results of the serological test, nucleic acid test and gene sequencing of the 68 samples all have indicated HIV-1 infection. Combined with the epidemiological data, it can be concluded that the double reaction of HIV-1 and HIV-2 antibodies in WB tests of these 68 samples is very likely to be a non-specific cross-reaction rather than HIV-2 infection. This study indicates that no HIV-2 infection cases have been found in Chengdu so far.
