Relationship between Follicular-fluid Neurotrophin-4， TrkB Receptors in Cumulus Cells and Oocyte Developmental Competence
- VernacularTitle:卵泡液中神经营养因子4及卵丘细胞TrkB受体与卵子发育潜能的关系
- Author:
Yi ZHOU
1
;
Ting-ting LI
1
;
Ying-chun GUO
1
;
Lei JIA
1
;
Shu-jing HE
1
;
Cong FANG
1
Author Information
1. Reproductive Medicine Center， The Sixth Affiliated Hospital， Sun Yat-sen University， Guangzhou 510655， China
- Publication Type:Journal Article
- Keywords:
neurotrophin-4 （NT-4）;
tropomyosin-related kinase B （TrkB） receptor;
follicular fluid （FF）;
cumulus cells （CCs）;
oocyte competence
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(2):257-265
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether the neurotrophin-4 （NT-4） in human follicular fluid （FF） and the expression of tropomyosin-related kinase B （TrkB） receptors in cumulus cells （CCs） have any correlation with the developmental competence of oocytes. MethodFF and CCs were collected from 63 patients undergoing intracytoplasmic sperm injection （ICSI） in the Reproductive Medicine Center from May to November 2020. NT-4 level in FF was measured by enzyme-linked immunosorbent assay （ELISA） method. The expression levels of two major TrkB isoforms， the full-length receptor （TrkB-fl） and the truncated receptor （TrkB-t1）， were quantified in CCs by real-time PCR. The correlation of oocyte maturation， fertilization and embryo development with NT-4 level in FF， TrkB-fl and TrkB-t1 expression in CCs were analyzed. ResultsNT-4 level in human FF was positively correlated with the number of normally fertilized oocytes （rs=0.250， P=0.048）， available embryos （rs=0.320， P=0.011）， top-quality embryos （rs=0.327， P=0.009） and top-quality blastocysts （rs=0.303， P=0.029）. The expression level of TrkB-t1 gene in CCs was negatively associated with a higher blastocyst formation rate （≥60%） and top-quality blastocyst rate （≥50%） ［0.86 （0.60， 1.85） vs. 2.29 （1.09， 3.44）， P=0.008； 0.84 （0.64， 1.45） vs. 1.73 （0.96， 3.14）， P=0.031］. Multiple linear regression analysis showed that the number of top-quality embryos was affected by the number of oocytes retrieved （P=0.001）， FF NT-4 level （P=0.005） and the expression of TrkB-t1 gene in CCs （P=0.049）. ConclusionFF NT-4 level was positively associated with oocyte developmental competence， and it might play an important role in the development of oocytes. Over-expressed TrkB-t1 in CCs might be related to compromised oocyte developmental potential.
