Impact of Autophagy in Vascular Smooth Muscle Cells on Mouse Aortic Dissection
- VernacularTitle:血管平滑肌细胞自噬对小鼠主动脉夹层形成的影响
- Author:
Lin CHEN
1
;
Ling-xia CHENG
1
;
Fan YANG
1
;
Ying LIU
1
;
Ying-chun HU
1
;
Wu ZHONG
1
Author Information
1. Department of Emergency Medicine， Affiliated Hospital of Southwest Medical University， Luzhou 646000， China
- Publication Type:Journal Article
- Keywords:
mouse;
aortic dissection;
autophagy;
vascular smooth muscle cell;
YAP
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(2):226-234
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the role of autophagy in vascular smooth muscle cells （VSMC） and the formation of aortic dissection （AD） using a murine model. Methods①Sixty C57BL/6 mice were randomly divided into three groups： control group， model group and chloroquine （CQ） group. The model and CQ groups were fed β-aminopropionitrile （BAPN） and injected with human angiotensin-Ⅱ （Ang-Ⅱ） for 16 days. The CQ group had chloroquine injected into the abdominal cavity of the mice daily while the control group was injected with normal saline using the same method. ②YAP， LC3， p62 and different phenotype related marker proteins were measured by western blot and immunohistochemistry staining. Results①The incidence of AD was 60% in the model group， while there was no case found in the CQ group. ②LC3 and synthetic related marker proteins of VSMC were significantly and highly expressed in the model and CQ groups， whereas the expression of VSMC contractile phenotype proteins and YAP inversely decreased（P<0.05）. ConclusionResults demonstrate that autophagy regulates the phenotypic transformation of VSMC via YAP protein pathways.
