Imperatorin Alleviates the Liver Damage in Young Rats with Streptozotocin-induced Type I Diabetes Mellitus
- VernacularTitle:白茅苷缓解STZ诱导的I型糖尿病幼龄鼠肝损伤
- Author:
Yan-qin DING
1
;
Xing-ping TANG
1
;
Kui XU
2
;
Ping LI
1
;
Ling LI
3
Author Information
1. Department of Paediatrics， Zhangye People's Hospital Affiliated to Hexi University ， Zhangye 734000，China
2. Diabetes Department of Zhangye Hospital of Traditional Chinese Medicine， Zhangye 734000，China
3. Office of Education and Science， Clinical Medical College of Hexi University， Zhangye 734000，China
- Publication Type:Journal Article
- Keywords:
imperatorin;
diabetes mellitus type 1;
oxidative stress;
immune response
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(2):218-225
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of imperatorin on liver damage， peroxidative stress and immune response in young rats with type I diabetes induced by streptozotocin （STZ）. MethodsTotally 50 BALB/c juvenile mice were equally divided into healthy control group， model group， model plus+3 mg/kg Imperatorin group， model+6 mg/kg Imperatorin group， and model+12 mg/kg Imperatorin group. Glucose tolerance test was used to detect fasting blood glucose， serum insulin and obesity index of mice in each group. Automatic biochemical analyzer was used to determine serum HDL， LDL， TG， TC content. HE staining was used to observe the degree of liver damage. TUNEL staining was used to observe cell apoptosis. SOD and MDA levels were detected by Kit， Box/Bcl-2， cleaved cas3/cas3， c-Myc expression levels in mitochondria by Western blot， and serum IL-6， TNF-α， IL-1β levels by ELISA. ResultsCompared with the model group， 12 mg/kg Imperatorin significantly reduced fasting blood glucose ［mean difference 5.3 mmol/L， 95% confidence interval （4.5， 6.1） mmol/L］， serum insulin ［mean difference 13.2 μU/L， 95% confidence interval （10.6， 15.8） μU/L］ and obesity index ［average difference 2.3 kg/m2， 95% confidence interval （2.1， 2.5） kg/m2］ all decreased. The content of SOD increased （P<0.05）， while the content of MDA was reduced （P<0.05）. The expression level of Bax/Bcl-2， and cleaved cas3/cas3 was down-regulated （P<0.05）， while the level of c-Myc was up-regulated （P<0.05）， and the content of IL-6， TNF-α and IL-1β was reduced （P<0.05）. ConclusionImperatorin can alleviate the liver damage， peroxidation and immune response in young rats with STZ-induced type I diabetes.
