Low-intensity Focused Ultrasound -triggered Intracellular Cavitation of Doxorubicin- loaded PLGA Nanoparticles Enhances Breast Cancer Cell Death
- VernacularTitle:低强度聚焦超声触发PLGA阿霉素在细胞内空化增加乳腺癌细胞死亡
- Author:
Pan-hui HUANG
1
;
Qiong LUO
2
;
Fei YAN
3
;
Shao-lin LI
1
Author Information
1. Department of Radiology， The Fifth Affiliated Hospital of Sun Yat-sen University， Zhuhai 519000， China
2. Department of Ultrasound Medicine， Guangzhou First People's Hospital， School of Medicine， South China University of Technology， Guangzhou 510180， China
3. Paul C. Lauterbur Research Center for Biomedical Imaging， Shenzhen Institute of Advanced Technology， Chinese Academy of Sciences， Shenzhen 518055， China
- Publication Type:Journal Article
- Keywords:
low-intensity focused ultrasound;
intracellular cavitation;
breast tumors;
cell death
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(2):209-217
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether low-intensity focused ultrasound can trigger the intracellular cavitation of the doxorubicin-loaded polylactic acid-glycolic acid copolymer （PLGA） nanoparticles and then enhance the death of 4T1 breast cancer cells. MethodsThe doxorubicin-loaded PLGA nano-bubbles were prepared by double emulsification solvent evaporation method. 4T1 breast cancer cells were divided into intracellular drug release group， extracellular drug release group， traditional drug delivery group， ultrasound alone group and control group. In each group， flow cytometry， cell counting kit-8 （CCK8） and scratch assay were used to detect the cell death， proliferation and invasion， respectively. ResultsThe average intensity of red fluorescence in the nuclei of the ultrasound group was higher than that of the control group and the difference was statistically significant （t=16.627， P<0.001）. The cumulative drug release of PLGA encapsulated doxorubicin at 3， 5， 7， 24 and 48 h in the ultrasound group were all higher than those in the control group and the differences were statistically significant （P<0.001）. The cell viability， cell proliferation and 24 h scratch pixel area in the intracellular drug release group， extracellular drug release group and traditional drug delivery group were all lower than those in the control group， with the results in the intracellular drug release group significantly lower than those in other groups. All the differences were statistically significant （P<0.001）. ConclusionsThe intracellular drug release triggered by low-intensity focused ultrasound through intracellular cavitation is more effective in enhancing the death of 4T1 tumor cells than the extracellular drug release triggered by low-intensity focused ultrasound and traditional drug delivery methods.
