Ferulic Acid Promotes Longevity and Stress Tolerance via the Insulin/IGF Signaling Pathway and DAF-16 in Caenorhabditis Elegans
XX.XXXX/j.issn.1672-3554.2021.02.005
- VernacularTitle:阿魏酸通过胰岛素/IGF信号通路和DAF-16促进秀丽隐杆线虫寿命和应激抵抗力
- Author:
Qi-fei KUANG
1
;
Qiao-chao CHEN
1
;
Ling ZHANG
1
;
Wen HE
1
;
Zhong PEI
2
Author Information
1. Department of Geriatrics，the First Affiliated Hospital of Sun Yat-sen University，Guangzhou 510080，China
2. Department of Neurology，the First Affiliated Hospital of Sun Yat-sen University，Guangzhou 510080，China
- Publication Type:Journal Article
- Keywords:
ferulic acid;
insulin/IGF signaling pathway;
DAF-16;
aging;
C. elegans
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(2):193-201
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect on life extension and the mechanism of ferulic acid in Caenorhabditis elegans. MethodsWe used C. elegans as an anti-aging model， and different concentrations of ferulic acid （0， 1， 10， 50 mmol/L） were given to explore its regulatory effects on the lifespan， anti-stress ability， and lipofuscin level ofC. elegans. And we used qPCR to screen senescence related genes， and DAF-2 （DR1572） and DAF-16 （CF1038） mutants were used to investigate the potential mechanism of ferulic acid. ResultsCompared with the blank control group， ferulic acid significantly extended the lifespan of C. elegans and increased its antioxidant and thermal stress （P＜0.001）， inhibited the accumulation of lipofuscin related to aging （P < 0.001）， and did not affect its fertility and body shape （P＞0.05） . After feeding nematode in the 50 mmol/L ferulic acid group， mRNA expression levels of DAF-2， Akt-2 and AGE-1 genes were down-regulated （P<0.05）， mRNA expression levels of DAF-16 genes were up-regulated （P＜0.05） and the expression levels of its downstream target genes sod-3， ctl-1， clk-2 and dod-17 were activated （P＜0.05）. After 50 mmol/L ferulic acid treatment， the proportion of daf-16 ：：GFP in the nematode cell nucleus increased from （5.3±1.5） % to （28±3） %. The lifespan of DAF-2 （DR1572） and DAF-16 （CF1038） mutant nematodes was not significantly extended after treatment with 50 mmol/L ferulic acid （P＞0.05）. ConclusionsFerulic acid can promote the nuclear localization of daf-16 through insulin /IGF signaling pathway， improve the stress resisting ability of the organism， and prolong the lifespan of nematode.
