Evaluation of The Effects of apoA1 on Retinal Angiogenesis of Mice in Vivo
- VernacularTitle:活体情况下评价载脂蛋白A1对小鼠视网膜血管生成的影响
- Author:
Kun-yi SU
1
;
Andina HU
1
;
Zhu-ting CHEN
1
;
Yu LIAN
1
;
Lin LV
1
;
Jie HU
1
Author Information
1. State Key Laboratory of Ophthalmology，Zhongshan Ophthalmic Center，Sun Yat-sen University，Guangzhou 510060，China
- Publication Type:Journal Article
- Keywords:
apoA1;
oxygen-induced retinopathy model;
blood-retinal barrier;
fundus fluorescein angiography
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(2):177-184
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveFundus imaging and fluorescein angiography were used to investigate the effect of apoA1 on the growth of retinal blood vessels under physiological environment and hypoxia state. MethodsUnder normal physiological conditions， by comparing apoA1 + / + mice and apoA1 - / - mice with C57 / BL6J mice， experiments in vivo were conducted at the end of the day 17 （infancy）， 8 weeks and 20 weeks， respectively， including： ① mice fundus photography （to observe vascular morphology） and ② fundus fluorescein angiography （FFA） （to observe vascular leakage， and to analyze average retinal vascular density， porosity and numbers of junction points）. Their OIR models were prepared respectively for experiments with fundus photography and FFA. ResultsUnder constant oxygen， fundus photography showed that the retinal blood vessels of the three kinds of mice were similar at different stages； FFA suggested that the capillary distribution of the three kinds of mice was uniform at different stages， and there was no obvious perfusion area and fluorescein leakage. There was no statistical difference between groups （Pvascular density =0.59 > 0.05， Pporosity =0.52 > 0.05）； Under hypoxia state， fundus photography showed that the main retinal vessels were obviously tortuous and dilated； FFA showed that all three groups had venous beaded changes， uneven capillary distribution， but no perfusion area and fluorescein leakage. Analysis of vascular density， porosity， and number of connection points showed there were significant differences between groups （Pvascular density =0.001 6 < 0.01， Pporosity =0.001 9 < 0.01， Pnumber of connection points =0.001 3< 0.01）. ConclusionsUnder physiological conditions， the expression level of apoA1 gene has no effect on retinal vascular growth. Under hypoxia state， increased apoA1 expression might reduce retinal nonperfusion area and reduce fluorescein leakage.
