Optimization of water extraction technology of Kaixin granules
- VernacularTitle:开心颗粒的水提工艺优化
- Author:
Zuomin WU
1
;
Shuxian BAI
1
;
Meng NING
1
;
Yunzhi WANG
1
;
Huifen MA
2
;
Jingyuan DONG
1
;
Zhongjie YANG
1
;
Zhishen XIE
2
;
Zhenqiang ZHANG
2
;
Xiaotao YU
1
Author Information
1. Dept. of Pharmacy，Luohe Central Hospital，Henan Luohe 462000，China
2. Academy of Chinese Medical Sciences，Henan University of Chinese Medicine，Zhengzhou 450046，China
- Publication Type:Journal Article
- Keywords:
Kaixin granules;
water extraction technology;
analytic hierarchy process;
entropy weight method;
grey relational
- From:
China Pharmacy
2025;36(22):2790-2795
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the water extraction technology for Kaixin granules. METHODS UPLC-MS/MS method was established for the simultaneous determination of ginsenoside Rg1， ginsenoside Re， ginsenoside Rb1， tenuifolin， polygalaxanthone Ⅲ and 3， 6′-disinapoyl-sucrose. An orthogonal test was designed with extraction times， extraction duration， and the volume of added water as factors. Using the contents of the aforementioned six indicator components and the extract yield as evaluation indexes， analytic hierarchy process-entropy weight method was employed to determine the combined weights of each indicator. Subsequently， process optimization and validation were conducted by integrating grey relational analysis （GRA） and back propagation （BP） neural network. RESULTS The water extraction technology optimized by the orthogonal test and GRA was 10- fold water for the first decoction and 8-fold water for the subsequent two， extracting 3 times，extracting for 1 h each time； the average comprehensive score of the validation experiment was 91.10 （RSD＝0.31%， n＝3）. The water extraction technology optimized by BP neural network was extracting 3 times with 10-fold water added each time， extracting for 1.5 h each time； the average comprehensive score of the validation experiment was 95.89 （RSD＝0.73%， n＝3）. Considering practical production requirements， the optimal water extraction technology was extraction performed three times， with 10-fold water for the first decoction and 8-fold water for the subsequent two extractions， with an extraction time of 1 h each. CONCLUSIONS The optimized water extraction technology for Kaixin granules is stable and feasible.
