Identification of Brucella strains in Ordos City, Inner Mongolia based on whole genome sequencing
- Author:
WANG Nana
;
JIA Yuan
;
WANG Sheng
- Publication Type:Journal Article
- Keywords:
Brucella;
whole genome sequencing;
species type identification;
Ordos
- From:
China Tropical Medicine
2025;25(3):333-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the whole genome structure of Brucella in Ordos city, conduct bioinformatics analysis, and explore a variety of Brucella species type identification methods, so as to provide a theoretical basis for the study of Brucella in Ordos City. Methods The whole genome was sequenced using illumina Miseq gene sequencer. The Microobench microbial gene analysis workstation was used to analyze the genome components, genome annotation, 16Sr DNA, SNP sites, insertions and deletions, MLST sites, and phylogenetic tree of 15 strains of Brucella. Results The average sequencing depth of 15 Brucella strains was 170.04×, the average genome size was 3 290 725 bp, the genome coverage was 99.93%, and the average GC content of assembly files was 54.69%. The results of 16Sr DNA analysis showed that the homology of 15 Brucella strains and Brucella Maltese was 100%. The analysis results of SNP sites and insert-deletion variation showed that the average number of SNPs of the 15 strains of Brucella was 2 412, the average number of polynucleotide mutations was 39, the average number of insertional variants was 131, the average number of missing variants was 153, the average number of complex variants was 43, and the average number of total variants was 2 778. MLST locus analysis showed that all 15 strains of Brucella were ST-8. Conclusion Based on the whole genome sequencing of 15 strains of Brucella in Ordos City, this study carried out a variety of type identification methods. Different methods have different focuses, and multiple methods are needed to confirm each other, so as to obtain more accurate experimental results and provide more research basis for the study of Brucella, to provide more data and theoretical support for the control and prevention of brucellosis in Ordos City.
- Full text:202511191641376793613.Identification of Brucella strains in Ordos City, Inner Mongolia based on whole genome sequencing.pdf
