To synthesise dual-action nanoparticles (nps) by integrating zno with the extract of urtica dioica l. And to characterise
- VernacularTitle:Цайрын оксидод суурилсан хоёр гэрт халгайн (urtica dioica l.) Хандтай нанопартиклыг гарган авсан дүн
- Author:
Odmaa T
1
;
Buyankhuu T
2
;
Oyun-Erdene R
2
;
Odgerel Ch
2
;
Tulgaa S
2
;
Otgonjargal Ch
2
;
Munkhtsetseg J
2
;
Nandin-Erdene M
3
Author Information
1. Bio-Medicine 505, School of Bio-Medicine, MNUMS
2. Department of Biochemistry, School of Bio-Medicine, MNUMS
3. Department of Biochemistry, School of Bio-Medicine, MNUMS;Institute of Biomedical Sciences, School of Bio-Medicine, MNUMS
- Publication Type:Journal Article
- Keywords:
Green synthesis;
bioactive compound;
characterization;
plant extract;
zinc oxide
- From:
Diagnosis
2025;113(2):45-52
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:The synthesis of multifunctional nanoparticles by integrating the bioactive properties of the ethanol extract of Urtica dioica L. a medicinal plant widely distributed in Mongolia, with zinc oxide nanoparticles (ZnO-NP) serves as the foundation of the present study. The aim is to produce nanoparticles with synergistic antioxidant, anti-inflammatory, antibacterial, and anticancer activities.
Objective:To synthesise dual-action nanoparticles (NPs) by integrating ZnO with the extract of Urtica dioica L. and to characterise their properties.
Materials and Methods:The Control group, as ZnO-NPs, and the study group, as medicinal plant ethanol extraction loaded nanoparticles (UD-ZnO-NPs), were synthesised using green synthesis techniques. The morphology and particle size were characterised by Scanning Electron Microscopy (SEM), chemical bonding was analysed using Fourier Transform Infrared Spectroscopy (FTIR), and crystalline structure was examined by X-ray Diffraction (XRD). Hemolytic activity assays were conducted to assess cytotoxicity.
Results:The Control and study group’s morphology and size distribution were uniform and spherical. The average particle size of the study group (UD-ZnO NPs) was 63 nm, while the control group (ZnO-NPs) was 77 nm. FTIR analysis showed that the basic chemical bonds in both types of nanoparticles were similar; however, additional peaks corresponding to the bioactive compounds from the Urtica dioica extract were detected in the UD ZnO-NPs. XRD analysis revealed that both types of ZnO-NPs investigated the same crystalline structure, consistent with the standard reference data (JCPDS No. 36 1451). Hemolysis assays showed that at concentrations ranging from 0.5 to 2.5 mg/ mL, the hemolytic activity was below 5%, indicating low cytotoxicity.
Conclusion:ZnO-NPs with and without Urtica dioica extract were successfully synthesized via a green method, yielding spherical, uniformly dispersed particles ranging from 63 to 77 nm in size. While the structural and crystalline characteristics of the NPs remained consistent, the presence of bioactive compounds was confirmed in the UD-ZnO-NPs. Hemolytic assays indicated dose-dependent cytotoxicity, highlighting the importance of concentration in biomedical applications.
- Full text:2025111416064044489Diagnosis-2025-113(2)-45-52.pdf
