Application value of nucleic acid mass spectrometry in detecting drug-resistance genes in Mycobacterium tuberculosis
- Author:
LI Aifang
;
CAO Junjie
;
LEI Jing
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
nucleic acid mass spectrum;
melting curve method;
drug resistance;
gene
- From:
China Tropical Medicine
2024;24(12):1529-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application value of nucleic acid mass spectrometry (MassARRAY) in the detection of drug resistance genes of Mycobacterium tuberculosis (MTB). Methods Pulmonary samples (sputum, bronchoalveolar lavage fluid) from suspected pulmonary tuberculosis patients and tissue and pus samples from suspected extrapulmonary tuberculosis patients were collected from Xi'an Chest Hospital from September 2022 to May 2023. Two samples from the same patient were subjected to both the melt curve method and the MassARRAY method to detect common drug resistance genes in patient samples, alongside mycobacterial culture and microplate drug susceptibility test. The efficacy of the Melt curve method and MassARRAY method for the detection of various drugs was evaluated using the microporous plate method as the standard. Results A total of 72 pulmonary specimens (40 sputa, 32 bronchoalveolar lavage fluids) and 36 extrapulmonary (18 tissues and 18 pus) were collected. The positive rate of mycobacterial culture was 32.4% (35/108). The detection rate of drug resistance genes by nucleic acid mass spectrometry was 52.8% (57/108). The resistance detection rates for rifampicin by nucleic acid mass spectrometry, melting curve, and microplate method were 14.0% (8/57), 13.0% (6/46), and 20.0% (7/35) respectively, with no significant difference among the three methods (F=0.851, P>0.05). There was no significant difference in the detection rates of isoniazid, ethambutol, streptomycin, and fluoroquinolones among the three methods. Using phenotypic drug susceptibility results of culture-positive samples as the reference standard, the Kappa values of rifampicin, isoniazid, ethambutol, and streptomycin detected by nucleic acid mass spectrometry were 1.000, 1.000, 0.635, and 0.821, respectively. Conclusions Nucleic acid mass spectrometry is a highly sensitive and specific method for detecting drug-resistance genes in Mycobacterium tuberculosis samples. It is a rapid, sensitive, accurate, and flexible molecular detection method with a promising application prospect in diagnosing drug-resistant tuberculosis.
- Full text:202511131527281451116.Application value of nucleic acid mass spectrometry in detecting drug-resistance genes in Mycobacterium tuberculosis.pdf
