Diagnostic value of miRNA-424-5p and IL-6 in different infection status of Mycobacterium tuberculosis
- Author:
HONG Mingyang
;
HUANG Xu
;
WANG Qiubo
;
ZHANG Haiyun
- Publication Type:Journal Article
- Keywords:
Latent tuberculosis infection;
active tuberculosis;
diagnosis;
microRNA;
IL-6
- From:
China Tropical Medicine
2024;24(12):1524-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in expression and diagnostic value of miRNA-424-5p and interleukin-6 (IL-6) in patients with latent tuberculosis (LTBI) and active tuberculosis (ATB). Methods Data from the miRNA expression microarray GSE25435 and GSE29190 were downloaded from the U.S. National Center for Biotechnology Information Gene Expression Omnibus (GEO) database, and differentially expressed miRNA were obtained using the GEO2R tool. A total of 30 cases, including LTBI and ATB patients, were selected from the Sixth People's Hospital of Nantong between July 2022 to July 2023, with healthy controls (HC) being concurrent healthy individuals. The expression of miRNA and IL-6 in peripheral blood mononuclear cells (PBMCs) among three groups was verified by real-time fluorogenic quantitative PCR (qPCR). The diagnostic efficacy of candidate miRNA and IL-6 was evaluated by receiver operating characteristic (ROC) curves. Results By GEO2R analysis, three differential miRNAs were obtained: miRNA-424-5p, miRNA-21-3p, and miRNA-144-3p. qPCR experiments verified the levels of these three miRNAs and IL-6, showing that miRNA-21-3p and miRNA-144-3p exhibited no significant difference in relative expression in PBMCs of ATB patients compared to the LTBI group (F=6.53, P>0.05; F=3.21, P>0.05). However, the expression levels of miRNA-424-5p and IL-6 in PBMCs were significantly increased, with statistically significant differences (F=5.35, P<0.01; F=8.77, P<0.01). ROC curve analysis showed that the maximum AUC of miRNA-424-5p and IL-6 for distinguishing ATB from LTBI was 0.70 and 0.76 respectively, and their combined diagnostic AUC could reach 0.84. Conclusions The combined diagnosis of miRNA-424-5p and IL-6 has the potential to distinguish LTBI from ATB patients, providing a certain reference for the rapid differential diagnosis of latent infection and active tuberculosis.
- Full text:202511131526193337015.Diagnostic value of miRNA-424-5p and IL-6 in different infection status of Mycobacterium tuberculosis.pdf
