Characterizing drug resistance and analyzing genetic evolution of Proteus mirabilis in a rural community
- Author:
ZHANG Yun
;
WANG Shuang
;
KOU Zengqiang
- Publication Type:Journal Article
- Keywords:
Proteus mirabilis;
drug resistance gene;
NDM-1;
whole genome sequencing
- From:
China Tropical Medicine
2024;24(11):1357-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular epidemiological characteristics of Proteus mirabilis from healthy populations, animals, and environmental sources in rural areas of Zhucheng, Weifang City, Shandong Province, China. Methods In this study, drug susceptibility testing was conducted on Proteus mirabilis isolated from healthy populations, animal feces, and sewage samples in Zhucheng, Weifang City, Shandong Province, China. The genetic environment surrounding the blaNDM-1 resistance gene was elucidated through whole-genome sequencing, and the genetic evolutionary relationships among strains were investigated through core genome multilocus sequence typing (cgMLST) and single nucleotide polymorphism (SNP) analysis. Results A total of 840 human fecal samples, 336 animal fecal samples, and 48 sewage samples were collected; the positivity rates were 3.21%, 0.89%, and 2.08%, respectively. Proteus mirabilis (PM) showed high sensitivity to meropenem (96.97%), ertapenem (96.97%), ceftazidime (84.85%), ceftazidime/avibactam (90.91%), and tigecycline (100%). A total of 54 antibiotic resistance genes were identified, with high detection rates of tet(J) (81.81%) and cat (81.81%). Two sewage-sourced PM strains carried both blaNDM-1 and blaOXA-1 resistance genes. The blaNDM-1 gene was flanked by IS91, ISVsa3, and IS6 insertion sequences. The sewage-derived isolates (E3DJ004, C3DJ004) and alleles such as 5pro, 16pro (2017, China, human) had only 69 SNP differences, indicating high genetic similarity. Strains (3BF020, 3BH05801) and (3BK028, 3BL044, 3BL045) were closely related (allelic and SNP differences <3), all of human origin, with SNP results consistent with cgMLST results. Conclusions Although PM in this study remains sensitive to carbapenems, it carries a greater number of drug-resistant genes. To prevent the undetected dissemination of drug-resistant genes, monitoring of multi-source samples from humans, animals, and the environment should be strengthened, to prevent the spread of drug-resistant bacteria at the "human-animal-environment" interface.
- Full text:202511121009120464310.Characterizing drug resistance and analyzing genetic evolution of Proteus mirabilis in a rural community.pdf
