Effects of laboratory domestication duration on the gut microbiota of Aedes aegypti
- Author:
HAN Wanrong
;
JIANG Yuting
;
GUO Xiaoxia
- Publication Type:Journal Article
- Keywords:
Aedes aegypti;
gut microbiota;
metagenome
- From:
China Tropical Medicine
2024;24(10):1279-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of laboratory domestication duration on the gut microbiota of mosquitoes, reveal the patterns of intestinal microbiota changes, and more systematically elucidate the role of mosquito gut microbiota on the transmission of vector-borne pathogens and mosquito resistance to insecticides, providing new insights into the interaction between microorganisms and mosquitoes. Furthermore, to understand the composition of gut microbiota in different geographic strains of mosquitoes to serve as a reference for selecting suitable populations for subsequent functional studies of gut microbiota. Methods The full-length bacterial ribosomal 16S amplification and metagenomic sequencing were performed to reveal the midgut microbiota in 5 Aedes aegypti strains which had been domesticated in the laboratory for various years. The composition, abundance, diversity, and correlation of the microbiota carried by the 5 mosquito strains were analyzed through bioinformatics, and the content of specific bacteria that play an important role in mosquito growth and development, pathogen transmission, and insecticide resistance in different samples were compared. Results The ML and CJ strains, which had been domesticated for a shorter duration, exhibited higher diversity indices compared to the DB and SA strains, which had been bred for a longer time. The gut microbiota of all mosquitoes mainly consisted of Proteobacteria, Firmicutes, and Bacteroidota. At the genus level, the bacterial composition of Aedes aegypti MH, DB, and SA strains was relatively homogenous, predominantly consisting of Cedecea and Asaia, while the ML and CJ strains, domesticated for a shorter period, harbored a richer and more balanced variety of bacteria. Conclusions Under laboratory conditions, the diversity of mosquito gut microbiota will decrease with increased domestication duration. Bacteria that have a positive effect on mosquito growth and development tend to be retained and increase in proportion over time, while the proportion of most other bacteria will decrease or even disappear.
- Full text:202511120950116061721.Effects of laboratory domestication duration on the gut microbiota of Aedes aegypti.pdf
