Significance of Th17Treg cells imbalance and changes in related cytokines expression levels of previous schistosomiasis patients
- Author:
WANG Huabing
;
ZHU Hong
;
ZHANG Xia
- Publication Type:Journal Article
- Keywords:
Schistosomiasis;
helper T cell 17;
regulatory T cells;
interleukin-17;
immune balance
- From:
China Tropical Medicine
2024;24(10):1243-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between the balance of helper T cell 17 (Th17)/regulatory T cell (Treg) and the expression levels of related cytokines such as interleukin 17 (IL-17), in key endemic areas of Hubei Province for previous schistosomiasis patients, to provide new targets for effective clinical assessment of the state of schistosomiasis. Methods A simple random sampling method was used to select previous schistosomiasis patients from Jiangling County, Jingzhou City, Hubei Province in 2023. And 15 cases were identified as the patient group (P group). Fifteen matched healthy individuals undergoing physical examination at the Hubei Provincial Center for Disease Control and Prevention in 2023 were selected as the control group (C group). The general and clinical data of the two groups were compared, along with the content of peripheral blood Th17, Treg, Th17/Treg ratio, and expression levels of related cytokines such as IL-17, IL-22, tumor necrosis factorα (TNF-α), interleukin 10 (IL-10), and transforming growth factor β1 (TGF-β1). The t-test was used to analyze the levels of Th17/Treg cell balance and related cytokines (e.g., IL-17) between the patient and control groups. Pearson correlation analysis was used for correlation analysis. Results There were no statistically significant differences between the two groups in terms of gender, age, height, and body mass (P>0.05). The serum biochemical indicators alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the patient group [(75.24±12.73) U/L, (61.96±11.82) U/L] were elevated compared to the control group [(18.42±1.21) U/L, (16.35±0.92) U/L], with statistically significant differences (P<0.05). The percentage of Th17 cells in the patient group was higher than that in the control group, and the Th17/Treg cell ratio was increased, with statistically significant differences (P<0.05). There was no statistically significant difference in the percentage of Treg cells between the two groups (P>0.05). The expression levels of IL-17 in peripheral blood were higher in the patient group compared to the control group, with a statistically significant difference (P<0.05), while the expression levels of IL-22, TNF-α, IL-10, TGF-β1 showed no statistically significant differences (P>0.05). The levels of Th17 (%) and cytokine IL-17 in the peripheral blood of the patient group were significantly positively correlated with ALT and AST (P<0.05). Conclusions Influenced by factors such as inflammatory reactions and immune dysfunction, the subjects of this study showed an increase in the Th17/Treg ratio of previous schistosomiasis patients, resulting in an imbalance of Th17/Treg cells and abnormal expression of cytokine IL-17. Therefore, detecting the expression of Th17/Treg cells and their related cytokines may provide a reference for the clinical assessment and symptomatic treatment of schistosomiasis.
- Full text:202511120931587158715.Significance of Th17Treg cells imbalance and changes in related cytokines expression levels of previous schistosomiasis patients.pdf
