Application of combined detection of Mycobacterium tuberculosis-specific cytokines IFN-γ and IL-2 in the diagnosis of active tuberculosis
- Author:
ZHANG Xin
;
CUI Xiaoli
;
WU Hang
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis-specific factor;
IL-2;
active tuberculosis;
clinical performance;
sensitivity
- From:
China Tropical Medicine
2024;24(9):1136-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic efficacy of combined detection of Mycobacterium tuberculosis-specific cytokines interferon-γ (IFN-γ) and interleukin-2 (IL-2) in active tuberculosis. Methods Data were collected from 292 inpatients at Xi'an Chest Hospital from June to December 2023, categorized into an active tuberculosis group with 160 cases (81 males and 79 females), and a non-tuberculosis group with 132 cases (72 males and 60 females). MTB-specific dual factor (IFN-γ and IL-2) detection, mycobacterial culture (BACTEC MGIT 960 liquid culture), real-time fluorescence quantitative reverse transcriptase-mediated PCR (qRT-PCR), simultaneous amplification and testing for Mycobacterium tuberculosis (SAT-TB), and sputum smear microscopy were performed on these patients. The diagnostic efficacy of MTB-specific double factorwas assessed, and differences in diagnosing active pulmonary tuberculosis using serological methods, bacteriological methods (mycobacterial culture, sputum smear microscopy), and molecular biological methods (qRT-PCR, SAT-TB) were compared. Results Using clinical diagnosis as a standard, the sensitivity, specificity, positive predictive value, negative predictive value, and Youden's index of MTB-specific dual factor detection were 80.63%, 80.30%, 83.23%, 77.37%, and 0.61, respectively. Compared with qRT-PCR, SAT-RNA, sputum smear microscopy, and mycobacterium culture, the sensitivity (χ2=66.117, 96.008, 103.789, 76.650; all P<0.001), Youden's index, and Kappa values of specific dual factor detection were higher than those of the other four detection methods, with no significant difference in specificity (χ2=1.918, 1.865, 0.000, 0.139, all P>0.05). In methodological comparisons, the serological group had a positive rate of 80.63%, higher than the bacteriological group (31.20%) and the molecular biological group (38.46%), with statistically significant differences (χ2=56.101, 74.645; both P<0.001). Conclusions The MTB-specific dual factor detection has a high sensitivity in the diagnosis of active tuberculosis and can be used as an immunological marker in the diagnosis of active tuberculosis, contributing to improving the accuracy of diagnosis.
- Full text:202511111557442086220.Application of combined detection of Mycobacterium tuberculosis-specific cytokines IFN-γ and IL-2 in the diagnosis of active tuberculosis.pdf
