Effect of NB-UVB irradiation on the expression of CCL20 in human HaCaT keratinocytes
- Author:
ZHENG XiaoLe
;
WANG Ping
;
YANG Zhili
- Publication Type:Journal Article
- Keywords:
Psoriasis;
NB-UVB;
immortalized human keratinocytes;
C-C motif chemokine ligand 20;
nuclear factor κB
- From:
China Tropical Medicine
2024;24(9):1112-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of narrow-band ultraviolet B (NB-UVB) irradiation, a psoriasis treatment, on the mRNA and proteinexpression of C-C motif chemokine ligand 20 (CCL20) in human immortalized keratinocytes (HaCaT cells) and to explore the underlying mechanisms. Methods HaCaT cells were divided into two groups after routine culture. The first group was exposed to NB-UVB at doses of 0, 100, 200, and 400 mJ/cm2, and treated after 3, 12, and 24 hours of culture. The second group was pre-treated with different concentrations of pyrrolidine dithiocarbamate ammonium (PDTC) (1.0, 12.5, 25.0 μmol/L), followed by NB-UVB (400 mJ/cm2) exposure. The expression of CCL20 mRNA in the cell culture lysate was detected using the reverse transcription polymerase chain reaction (RT-PCR) method, the expression of CCL20, nuclear factor κB(NF-κB) p65, and phosphorylated NF-κB p65 proteins in HaCaT cells was detected by Western blot, and the expression of CCL20 protein in the cell culture supernatant was measured by enzyme-linked immunosorbent assay (ELISA). Results After NB-UVB irradiation, the CCL20 mRNA expression level in HaCaT cells and the CCL20 protein expression level in cell culture supernatant increased with the increase of NB-UVB irradiation dose, showing a statistically significant difference (P<0.05). The phosphorylation level of NF-κB p65 in HaCaT cells also increased with the increase of NB-UVB irradiation dose, demonstrating a statistically significant difference (P<0.05). Compared with the group treated solely with NB-UVB, the mRNA and protein expression levels of CCL20, as well as the phosphorylation level of NF-κB p65 in HaCaT cells pre-treated with PDTC, were all reduced to varying degrees (P<0.05), and exhibited a dose-dependent manner, with a more pronounced reduction as the concentration of PDTC increased. Conclusions NB-UVB can regulate the expression and secretion of the chemokine CCL20 in human HaCaT keratinocytes through the activation of the NF-κB signaling pathway.
- Full text:202511111548548678716.Effect of NB-UVB irradiation on the expression of CCL20 in human HaCaT keratinocytes.pdf
