Evaluation of changes in human salivary amylase enzyme activity
- VernacularTitle:Хүний шүлсний амилаза энзимийн өөрчлөлтийг үнэлэх нь
- Author:
Sarangerel G
1
;
Odmaa T
1
;
Buyankhuu T
2
;
Oyun-Erdene P
2
;
Odgerel Ch
2
;
Tulgaa S
2
;
Otgonjargal Ch
2
;
Munkhtsetseg J
2
;
Nandin-Erdene M
3
Author Information
1. School of Biomedicine, MNUMS
2. Department of Biochemistry, School of Biomedicine, MNUMS
3. Institute of Biomedicine, School of Biomedicine, MNUMS;Department of Biochemistry, School of Biomedicine, MNUMS
- Publication Type:Journal Article
- Keywords:
carbohydrate;
non-invasive;
biomarker;
protein;
blood
- From:
Diagnosis
2025;114(3):104-111
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Saliva as a non-invasive biological sample can be a game-changer in early disease detection and health risk assessment.
Objective:To examine the association between participants' dietary patterns and the activity of salivary amylase, along with serum amylase levels in humans.
Materials and methods:This study was conducted at the research laboratory of the Department of Biochemistry, School of Biomedicine, MNUMS. A total of 30 students aged 19–22 years participated in the study. Saliva samples were collected three times at one-week intervals, and one blood sample was collected from each participant, alongside a dietary questionnaire. The activity of the amylase enzyme in both saliva and serum samples was determined using the iodine-starch method.
Results:When evaluating the amylase enzyme activity based on participants' carbohydrate intake, the result was p > 0.05, indicating no statistically significant difference. Similarly, statistical analysis of the use of mouthwash and vitamin supplements also showed p > 0.05, which means these variables had no statistically significant effect on amylase activity. The correlation between salivary and serum amylase activity was found to be r = 0.365, indicating a weak positive correlation, but the difference was not statistically significant.
Conclusion:The intake of carbohydrates, vitamins, and mouthwash does not significantly affect the activity level of the salivary amylase enzyme, according to research findings. However, external factors such as stress and air pollution have been shown to exert a measurable influence on its activity. A comparative analysis of enzyme levels in saliva and blood using amylase as a representative marker revealed similar activity levels in both fluids. This suggests that saliva may serve as a viable non invasive sample for detecting various biomarkers and diagnosing diseases. The results underscore the potential of salivary components, particularly amylase, as valuable indicators in diagnostic applications.
- Full text:2025111114262344723Diagnosis-2025-114(3)-104-111.pdf
