Correlation Analysis of Eya3 with Prognosis and Tumor Immune Microenvironment in Esophageal Cancer
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2025.0514
- VernacularTitle:Eya3与食管癌患者预后及肿瘤免疫微环境的相关性分析
- Author:
Jingjing CHENG
1
;
Danyu GUO
;
Dong QIAN
;
Jieyong TIAN
Author Information
1. 中国科学技术大学附属第一医院//安徽省立医院肿瘤放疗科,安徽 合肥 230001;安徽省肿瘤医院肿瘤放疗科,安徽 合肥 230031
- Publication Type:Journal Article
- Keywords:
esophageal carcinoma;
neoadjuvant chemoradiation;
Eya3;
CD8+T lymphocytes;
antitumor immunity
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(5):843-850
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the association between Eya3 expression and CD8+T cell infiltration levels in the tumor microenvironment,as well as their prognosis in esophageal carcinoma(EC)patients following neoadjuvant chemoradiation(neo-CRT).[Methods]The pathological specimens before treatment and after surgery and clinical data were collected from 50 patients with EC who received neo-CRT combined with surgery.The correlation between the Eya3 expression and postoperative pathological features,CD8+T cell infiltration levels,disease-free survival(DFS)and overall survival(OS)were analyzed using immunohistochemistry(IHC)and multicolor immunofluorescence staining.[Results]The rate of pathological complete response(pCR)was higher in patients with low baseline Eya3 expression in the EC before treatment(P=0.001 8).Both median DFS(37.2 months vs.15.3 months,HR:2.731,95%CI:1.167-6.394,P=0.021)and median OS(not reached vs.26.7 months,HR:3.959,95%CI:1.539-10.18,P=0.004 3)are significantly longer for patients with low Eya3 than those with high Eya3.The expression of Eya3 significant negatively correlated with tumor-infiltrating CD8+T lymphocytes(P＜0.001),GZMB+CD8+T lymphocytes P=0.002)in surgical pathological specimens.The patients with high CD8+T lymphocytes and GZMB+CD8+T lymphocytes had significantly longer DFS than those with low level.[Conclusion]Patients with EC who have high expression of Eya3 have a poorer prognosis,and the infiltration levels of CD8+T lymphocytes and GZMB+CD8+T lymphocytes in the tumor microenvironment are lower.This suggests that the expression of Eya3 may affect the tumor immune microenvironment and thereby influence the prognosis of EC patients,and it may become a molecular marker for predicting the prognosis of EC patients..
