Correlation Analysis of Eya3 with Prognosis and Tumor Immune Microenvironment in Esophageal Cancer
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2025.0514
- VernacularTitle:Eya3与食管癌患者预后及肿瘤免疫微环境的相关性分析
- Author:
Jingjing CHENG
1
;
Danyu GUO
1
;
Dong QIAN
1
;
Jieyong TIAN
2
Author Information
1. Department of Radiation Oncology， the First Affiliated Hospital of USTC // Anhui Provincal Hospital， Hefei 230001， China
2. Department of Thoracic Surgery， the First Affiliated Hospital of USTC // Anhui Provincal Hospital， Hefei 230001， China
- Publication Type:Journal Article
- Keywords:
esophageal carcinoma;
neoadjuvant chemoradiation;
Eya3;
CD8+ T lymphocytes;
antitumor immunity
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(5):843-850
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the association between Eya3 expression and CD8+ T cell infiltration levels in the tumor microenvironment， as well as their prognosis in esophageal carcinoma （EC） patients following neoadjuvant chemoradiation （neo-CRT）. MethodsThe pathological specimens before treatment and after surgery and clinical data were collected from 50 patients with EC who received neo-CRT combined with surgery. The correlation between the Eya3 expression and postoperative pathological features， CD8+ T cell infiltration levels， disease-free survival（DFS） and overall survival （OS） were analyzed using immunohistochemistry（IHC） and multicolor immunofluorescence staining. ResultsThe rate of pathological complete response （pCR） was higher in patients with low baseline Eya3 expression in the EC before treatment（P=0.001 8）. Both median DFS （37.2 months vs. 15.3 months， HR：2.731，95% CI：1.167-6.394，P=0.021） and median OS （not reached vs. 26.7 months， HR：3.959，95%CI：1.539-10.18，P=0.004 3） are significantly longer for patients with low Eya3 than those with high Eya3. The expression of Eya3 significant negatively correlated with tumor-infiltrating CD8+T lymphocytes （ P＜0.001）， GZMB+CD8+T lymphocytes P=0.002） in surgical pathological specimens . The patients with high CD8+T lymphocytes and GZMB+CD8+T lymphocytes had significantly longer DFS than those with low level. ConclusionPatients with EC who have high expression of Eya3 have a poorer prognosis， and the infiltration levels of CD8+T lymphocytes and GZMB+CD8+T lymphocytes in the tumor microenvironment are lower. This suggests that the expression of Eya3 may affect the tumor immune microenvironment and thereby influence the prognosis of EC patients， and it may become a molecular marker for predicting the prognosis of EC patients..
