Dynamics Changes of Long Non-Coding RNA in the Testis and Epididymis During Male Mouse Aging
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2025.0510
- VernacularTitle:雄性小鼠衰老过程中睾丸和附睾长链非编码RNA的动态变化
- Author:
Hongle HE
1
;
Rui SUN
1
;
Jinhong GUAN
2
;
Ruoyang CHEN
2
;
Yun XIE
1
;
Chengqiang MO
1
;
Xianshen SHA
3
;
Yanping HUANG
4
Author Information
1. Department of Urology， The First Affiliated Hospital of Sun Yat-Sen University // Guangdong Provincial Clinical Research Center for Urological Diseases， Guangzhou 510080， China
2. Zhongshan School of Medicine， Sun Yat-sen University， Guangzhou 510080， China
3. Department of Urology， The First Affiliated Hospital of Jinan University， Guangzhou 510632， China
4. Department of Urology and Andrology， Renji Hospital， Shanghai Jiao Tong University School of Medicine//Shanghai Institute of Andrology， Shanghai 200001， China
- Publication Type:Journal Article
- Keywords:
long non-coding RNAs;
male reproductive tract;
reproductive aging;
testis;
epididymis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(5):806-815
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the dynamic expression profiles and potential regulatory mechanisms of long non-coding RNAs （lncRNAs） in male reproductive system aging. MethodsA naturally aging C57BL/6 mouse model was used and 4 mice were selected each at 3， 15， and 21 months of age. RNA was extracted from seven regions of the male reproductive tract （testis， efferent duct， initial segment of epididymis， caput epididymis， corpus epididymis， cauda epididymis， and vas deferens）， followed by RNA sequencing and bioinformatics analysis. ResultsRegion-specific dynamic expression profiles of lncRNAs were constructed in the testis， epididymis （efferent duct， initial segment， caput， corpus， and cauda）， and vas deferens of male mice. Combined with gene functional enrichment analysis， the functional associations of lncRNAs were elucidated in reproductive system aging. The differentially expressed lncRNAs in the aging testis were primarily involved in hormone biosynthesis and extracellular matrix organization， while those in the initial segment of the epididymis were closely related to cell recognition and epithelial cell migration. A comprehensive lncRNA expression atlas associated with male reproductive aging was established. ConclusionLncRNAs may participate in male reproductive aging through the regulation of the reproductive microenvironment， which provides key molecular targets and a research foundation for understanding age-related fertility decline.
