Quality evaluation of Jianggui granules by HPLC fingerprint combined with chemometrics
10.12206/j.issn.2097-2024.202312040
- VernacularTitle:应用HPLC指纹图谱结合化学计量学对姜桂颗粒质量的评价研究
- Author:
Bei HU
1
;
Yaling CUI
1
;
Liangyu MA
1
;
Zihua XU
1
Author Information
1. Department of Pharmacy, General Hospital of Northern Theater Command of PLA, Shenyang 110000, China.
- Publication Type:Originalarticles
- Keywords:
Jianggui granules;
fingerprint;
HPLC;
similarity evaluation;
chemometrics
- From:
Journal of Pharmaceutical Practice and Service
2025;43(10):509-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the fingerprint of Jianggui granules, and evaluate it by chemometrics. Methods The fingerprint of Jianggui granules was established by HPLC. Similarity evaluation system of chromatographic fingerprint of TCM （2012 edition） was used to evaluate the similarity evaluation. Then, the quality of the drug was assessed by cluster analysis （CA）, principal components analysis （PCA） and partial least squares discriminant analysis （PLS-DA）. Results The characteristic fingerprint of Jianggui granules was established and 18 common peaks were verified. Five chromatographic peaks were identified，i.e. Puerarin, glycyrrhizin, cinnamic acid, cinnamaldehyde and ammonium glycyrrhizinate. The similarities of samples were >0.9. Results of CA showed that 14 batches of samples could be classified into two categories：S1 and S4 were grouped into one category；others were grouped into the other category. The results of PCA showed that the cumulative contribution rate of the first two principal components was 96.61%. The results of OPLS-DA showed that the eleven peaks with VIP value >1 were puerarin （peak 8）, glycyrrhizin （peak 14）, cinnamaldehyde （peak 17） and ammonium glycyrrhizinate （peak 18）. Conclusion HPLC fingerprint of Jianggui granules was established. The established method was accurate and reliable，which could be used in quality evaluation of Jianggui granules.
