Ameliorative effect of patchouli alcohol on mice with lung-heat syndrome based on PI3K/Akt/NF-κB pathway
10.3969/j.issn.1006-2157.2025.04.003
- VernacularTitle:基于PI3K/Akt/NF-κB通路研究百秋李醇对肺热证小鼠的改善作用
- Author:
Linze LI
1
;
Yi LI
1
;
Haoyi QIAO
1
;
Jiakang JIAO
2
;
Qi ZHANG
1
;
Xiaofang WU
1
;
Xingyu ZHAO
1
;
Yinming ZHAO
1
;
Chun WANG
2
;
Jianjun ZHANG
2
;
Linyuan WANG
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine
2. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- From:
Journal of Beijing University of Traditional Chinese Medicine
2025;48(4):459-470
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the therapeutic effect of patchouli alcohol on mice with lung-heat syndrome based on the phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)/nuclear factor-kappa B(NF-κB) signaling pathway.
Methods:First, network pharmacology was used to predict the potential targets of patchouli alcohol in the treatment of lung-heat syndrome, and a "component-disease-key target" network was constructed for pathway analysis. Then, 40 BALB/c mice were assigned to the normal, lung-heat model, honeysuckle, and low-dose and high-dose patchouli alcohol groups. All groups, except the blank group, were intranasally infected with 50 μL (103 TCID50) of influenza virus solution. After two hours of infection, mice were treated once a day for seven consecutive days. The therapeutic mechanism of patchouli alcohol was explored by measuring pulmonary inflammatory factors, the PI3K/Akt/NF-κB pathway, hypothalamic fever markers (PGE2, cAMP, cGMP levels), rectal temperature, and tissue energy metabolism.
Results:Network pharmacology identified 135 target genes related to patchouli alcohol and lung-heat syndrome, with the key targets being STAT3, H1F1A, and NF-κB1. In animal experiments, patchouli alcohol significantly alleviated influenza virus-induced lung inflammatory damage in mice with lung-heat syndrome, inhibited the expression of TNF-α and IL-6 in lung tissues(P<0.01), and suppressed the activation of the PI3K/Akt/NF-κB pathway. It also reduced hypothalamic levels of PGE2 and cAMP(P<0.01), suppressed the increase in rectal temperature, significantly decreased liver glycogen and pyruvate levels(P<0.01), and increased the activities of SDH, LDH, and Na+ -K+ -ATPase in the liver(P<0.01)
Conclusion:Patchouli alcohol improves the symptoms of lung-heat syndrome in mice by inhibiting the activation of the PI3K/Akt/NF-κB pathway, reducing proinflammatory cytokines and inflammatory damage, and regulating hypothalamic fever markers and energy metabolism.
