Isolation, identification, and mouse safety evaluation of bovine-derived Pediococcus acidilactici
10.13200/j.cnki.cjb.004570
- VernacularTitle:1株牛源乳酸片球菌的分离、鉴定及其小鼠安全性评价
- Author:
WANG Minglu
- Publication Type:Journal Article
- Keywords:
Bovine rumen;
Pediococcus acidilactici;
Growth characteristics;
Probiotic characteristics;
Safety
- From:
Chinese Journal of Biologicals
2025;38(10):1187-1193
- CountryChina
- Language:Chinese
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Abstract:
Objective To isolate a strain from rumen contents of healthy cattle, and evaluate its growth and probiotic properties as well as its safety in mice in vitro.Methods The microbial classification of this strain was determined by Gram staining, physiological and biochemical experiments combined with 16S rRNA sequencing. The growth, probiotic characteristics, and safety of the strain were evaluated through its growth curve, acid production curve, antibiotic sensitivity, pH,artificial gastrointestinal environment and high temperature tolerance, as well as in vitro experiments in mice.Results After morphological, physiological and biochemical identification and 16S rRNA sequencing analysis, the isolate was identified as Pediococcus acidilactici, named BoP2312. BoP2312 grew to a stable stage after 8 h of growth, and the final pH of the bacteria solution stabilized at 3. 6. The survival rates in artificial gastric juice and intestinal juice were above(53. 42 ± 0. 013)% and(98. 22 ± 0. 003)%, respectively, and it could be tolerated for 10 min at 60 ℃. The strain could inhibit the growth of common pathogenic bacteria and was insensitive to antibiotics such as polymyxin B, norfloxacin and ciprofloxacin. There was no abnormal performance of mice in the safety test. The body weight gain of mice in the group administered with Pediococcus acidilactici by gavage was significantly higher than that of the control group(t = 6. 913, P < 0. 01), and the ratio of feed to gain was significantly lower than that in the control group(t = 3. 74, P < 0. 05). Intragastric administration of Pediococcus acidilactici could decrease the activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum of mice(t = 2. 737 and 1. 321, P = 0. 016 and 0. 209, respectively).Conclusion Pediococcus acidilactici BoP2312 isolated from the rumen of healthy cattle has good growth and probiotic properties, and was safe to mice, which could be used as a candidate for the further study of bovine-derived probiotics.
