Multivariate quantitative combined with chemometrics for evaluating the quality of Sophora flavescens from different producing areas
- VernacularTitle:多指标定量联合化学计量学评价不同产地苦参质量
- Author:
Jiahui CHEN
1
;
Qiong LUO
1
;
Junli ZHAO
2
;
Yan HAI
2
;
Chengdong LIU
3
;
Tuya BAI
4
;
Jun LI
4
;
Yuewu WANG
3
Author Information
1. Dept. of Pharmacy，Baotou Third Hospital，Inner Mongolia Baotou 014040，China
2. Dept. of Pharmacy，Baotou Central Hospital，Inner Mongolia Baotou 014040，China
3. School of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China
4. School of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China;Inner Mongolia Engineering Research Center for New Drug Screening，Hohhot 010110，China
- Publication Type:Journal Article
- Keywords:
Sophora flavescens;
quality assessment;
HPLC-MS/MS method;
TOPSIS;
grey relational analysis
- From:
China Pharmacy
2025;36(19):2404-2408
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a content determination method for multiple components in Sophora flavescens from different origins and to evaluate its quality by combining with chemometrics. METHODS Thirteen batches （No. K1-K13） of S. flavescens from different origins were selected as test samples. A high-performance liquid chromatography-tandem triple quadrupole mass spectrometry （HPLC-MS/MS） method was established to determine the contents of 12 components， including matrine， oxymatrine， betaine， cytisine， N-methylcytisine， sophoridine， genistein， sophoricoside， sophorone， formononetin， sophorolone Ⅰ and norkurarinone in S. flavescens. Chromatographic separation was performed on a Shim-pack GIST-HP C18 column with a mobile phase consisting of methanol （A） and water containing 0.1% formic acid （B）， using gradient elution at a flow rate of 0.25 mL/min， column temperature of 35 ℃， and an injection volume of 3 μL. Mass spectrometry was conducted using an electrospray ionization source with positive and negative ion scanning. Data were collected in segments using the multiple reaction monitoring mode. Technique for order preference by similarity to ideal solution （TOPSIS） and grey relational analysis （GRA）methods were employed to compare and comprehensively evaluate the 13 batches of S. flavescens from different origins. RESULTS The methodological validation for the content determination met the relevant regulatory requirements. The contents of the 12 components were 490.66-1 231.00， 11 088.10- 18 021.50， 7.91-25.38， 903.97-1 713.64， 336.08-1 485.54，1 065.33-2 075.50， 27.52-71.80， 109.36-517.83， 6 034.55-10 632.73， 21.26-145.35， 814.84-1 911.32， 1 040.87-3 446.37 μg/g）， respectively. TOPSIS results showed that the top 7 samples in Euclidean distance ranking were K6， K12， K11， K3， K5， K10， K13. The GRA results showed that the top 7 samples in the relative correlation ranking were K12， K11， K10， K6， K13， K5， K3. CONCLUSIONS The established HPLC-MS/MS method is rapid， accurate， highly sensitive， stable and reliable. Combined with chemometrics methods， it can be used for the quality control and evaluation of S. flavescens. The comprehensive quality of samples K3， K5， K6（ from Hebei）， K10（ from Sichuan）， K11-K13（ from Shanxi）， etc. is relatively superior.
