Analysis of red blood cell blood group gene polymorphism and applicability evaluation of PCR-TaqMan technology in the Hui ethnic blood donor population in Suzhou
10.13303/j.cjbt.issn.1004-549x.2025.09.015
- VernacularTitle:苏州回族献血人群红细胞血型基因多态性分析与PCR-TaqMan技术适用性评估
- Author:
Jia JIANG
1
;
Zhihong FANG
2
;
Zihao XU
1
;
Kai WANG
1
;
Nina JIANG
1
Author Information
1. Suzhou Blood Center, Suzhou 215008, China
2. Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou 215008, China
- Publication Type:Journal Article
- Keywords:
rare blood group;
gene frequency;
real-time PCR;
Suzhou
- From:
Chinese Journal of Blood Transfusion
2025;38(9):1218-1224
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the characteristics of allele frequencies for 9 red blood cell (RBC) blood group systems in the Hui ethnic voluntary blood donor population of Suzhou using real-time fluorescence PCR technology, so as to provide technical support for establishing a RBC blood group genetic database. Methods: PCR-TaqMan technology was employed to perform genotyping detection for 9 RBC blood group systems using 144 samples from Hui voluntary blood donors in Suzhou, collected between October 2023 and August 2024. Results: Blood group allele frequencies among Suzhou Hui voluntary blood donors were distributed as follows: MNS system (M=0.566 0, N=0.434 0; S=0.079 9, s=0.920 1); Lutheran system (Lu
=0.003 5, Lu
=0.996 5; Au
=0.895 8, Au
=0.104 2); Kell system (K=0.000 0, k=1.000 0; Kp
=0.003 5, Kp
=0.996 5; JS
=0.000 0, JS
=1.000 0); Duffy system (Fy
=0.899 3, Fy
=0.100 7); Kidd system (JK
=0.451 4, JK
=0.548 6); Diego system (Di
=0.041 7, Di
=0.958 3); Yt system (Yt
=0.996 5, Yt
=0.003 5); Dombrock system (Do
=0.128 5, Do
=0.871 5); Colton system (Co
=1.000 0, Co
=0.000 0). The PCR-TaqMan-based RBC blood group genotyping technology successfully completed testing for all samples. Conclusion: The MNS, Lutheran, Duffy, Kidd, Diego, and Dombrock blood group systems in the Suzhou Hui population exhibited polymorphic distribution patterns, whereas the Colton system was monomorphic. Standardized application of PCR-TaqMan technology facilitates the establishment of an RBC blood group genetic database.
