Study on the role of toll-like receptor 3 in RhD alloimmunization
10.13303/j.cjbt.issn.1004-549x.2025.09.007
- VernacularTitle:Toll样受体3在RhD同种免疫发生中的作用研究
- Author:
Sitian CHEN
1
;
Li TIAN
2
;
Ning SONG
2
;
Zhong LIU
2
Author Information
1. Laboratory Center, Hunan Children's Hospital, Changsha 410007, China; Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China; Key Laboratory of Transfusion Adverse Reactions, Chinese Academy of Medical Sciences, Chengdu 610052, China
2. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China; Key Laboratory of Transfusion Adverse Reactions, Chinese Academy of Medical Sciences, Chengdu 610052, China
- Publication Type:Journal Article
- Keywords:
toll-like receptor 3;
RhD alloimmunization;
red blood cell transfusion;
interferon-alpha
- From:
Chinese Journal of Blood Transfusion
2025;38(9):1167-1171
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To preliminarily investigate the role of toll-like receptor 3 (TLR3) in RhD alloimmunization. Methods: Between January 10, 2018 and April 1, 2019, RhD negative plasma donors were recruited for active RhD alloimmunization. A panel of four RhD 15-mer peptides was synthesized to stimulate T-cell proliferation. Peripheral blood mononuclear cells (PBMCs) were collected from successfully immunized donors (responders) and divided into an experimental group (pre-incubated with anti-TLR3 antibody before adding RhD peptides) and a control group (RhD peptides only). PBMCs proliferation was assessed using a cell proliferation enzyme-linked immunosorbent assay (BrdU), while TLR3 and IFN-α levels in cell culture supernatants and serum were detected via ELISA. Results: PBMCs proliferation was significantly lower in the experimental group compared to the control group [(31 234±15 300) vs (61 225±25 073) rlu/s, P<0.001]. TLR3 expression levels in the cell culture supernatants were also reduced in the experimental group [(3.49±0.32) vs (4.01±0.35) ng/mL, P<0.001]. TLR3 protein levels were detectable in responders but undetectable in the serum of non-responders [(1.43±1.44) vs 0 ng/mL, P<0.001]. A strong linear correlation was observed between TLR3 and IFN-α levels in serum, described by the regression model Y=2.957X+1.004 (r
=0.91, P<0.001). Conclusion: TLR3 activation may promote RhD alloimmunization by inducing IFN-α secretion, indicating a critical role of the TLR3 signaling pathway in RhD immune responses.
