Comparative analysis of characteristics and functions of exosomes from human induced pluripotent stem cell-derived platelets and apheresis platelets
10.13303/j.cjbt.issn.1004-549x.2025.09.005
- VernacularTitle:人诱导多能干细胞来源血小板外泌体与单采血小板外泌体特征及功能差异性研究
- Author:
Weihua HUANG
1
;
Yan ZANG
1
;
Aihua QIN
1
;
Ziyang FENG
1
;
Heshan TANG
1
;
Fei GUO
1
;
Chuyan WU
1
;
Qiu SHEN
1
;
Baohua QIAN
1
;
Haihui GU
1
;
Zhanshan CHA
1
Author Information
1. Department of Transfusion Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China
- Publication Type:Journal Article
- Keywords:
human induced pluripotent stem cells;
apheresis;
platelets;
exosomes;
human umbilical cord mesenchymal stem cells
- From:
Chinese Journal of Blood Transfusion
2025;38(9):1154-1161
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the biological characteristics of human induced pluripotent stem cell-derived platelet exosomes (hiPSC-Plt-Exos) with those of conventional apheresis platelet exosomes (Plt-Exos), specifically focusing on their differential abilities to enhance the proliferation and migration of human umbilical cord mesenchymal stem cells (hUC-MSCs). Methods: Exosomes were isolated from hiPSC-derived Plt and apheresis Plt concentrate using size exclusion chromatography. These exosomes were then characterized through nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and Western blotting. Co-culture experiments into hUC-MSCs were conducted with hiPSC-Plt-Exos and apheresis Plt-Exos, respectively. Their effects on the proliferation and migration of hUC-MSCs were assessed via cell proliferation assays and scratch tests. Results: hiPSC-Plt-Exos and apheresis Plt-Exos exhibited comparable particle sizes, morphological features (such as the characteristic cup-shaped structure), and surface markers (including CD9 and HSP70). Notably, hiPSC-Plt-Exos demonstrated a significantly greater ability to enhance the proliferation and migration of hUC-MSCs compared to apheresis Plt-Exos (P<0.05). These differences provide critical comparative data for their application in various clinical contexts. Conclusion: This study establishes a theoretical foundation for developing precise therapeutic strategies based on hiPSC-Plt-Exos. Furthermore, it underscores the necessity of selecting the appropriate type of exosomes according to the specific disease microenvironment to achieve optimal therapeutic outcomes.
