Evaluation of the quality of Alpinia katsumadai from different habitats based on HPLC fingerprint and determina-tion of active ingredient content
- VernacularTitle:基于HPLC指纹图谱和有效成分含量评价不同产地草豆蔻质量
- Author:
Yanwu LIAO
1
;
Yingjun WANG
1
;
Shun LIU
2
;
Mingming QIU
1
;
Yufeng ZHU
1
Author Information
1. Dept. of Pharmacy，the Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine，Nanjing 210029，China
2. Jiangsu Engineering Research Centre for Innovative Chinese Medicine Preparations，Nanjing 210029，China;The First Clinical Medical College，Nanjing University of Chinese Medicine，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
Alpinia katsumadai;
HPLC fingerprint;
chemical pattern recognition;
content determination;
habitats;
quality control
- From:
China Pharmacy
2025;36(18):2262-2266
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the quality differences of Alpinia katsumadai from different habitats. METHODS High- performance liquid chromatography（HPLC） was used to establish the fingerprints of A. katsumadai from 18 batches of different habitats， and the quality of A. katsumadai from different habitats was comprehensively evaluated by similarity evaluation， cluster analysis （CA）， principal component analysis （PCA）， orthogonal partial least squares-discriminant analysis （OPLS-DA） and the content determination results of alpinetin， pinocembrin， cardamonin and alnustone in A. katsumadai. RESULTS The similarity of HPLC fingerprints for 18 batches of A. katsumadai was ＞0.9. Eleven common peaks were identified from the chromatogram， and four of them were specifically characterized. Both CA and PCA grouped 18 batches of A. katsumadai into 3 categories， extracting 2 principal components （the cumulative variance contribution rate reached 89.798%）. OPLS-DA identified 9 quality difference markers， namely the components corresponding to peaks 4， 9， 3， 2， 7 （pinocembrin）， 8 （cardamonin）， 6 （alpinetin）， 10 and 11 （alnustone）. The content of alpinetin， pinocembrin， cardamonin， and alnustone ranged from 4.507 1-11.579 7， 5.154 4-14.183 3， 5.109 5-13.588 3 and 4.494 6-11.277 2 mg/g， respectively. CONCLUSIONS The quality of A. katsumadai from different habitats is quite different， and the quality of A. katsumadai from Hainan is the best.
