Difference in Resistance to Streptococcus pneumoniae Infection in Mice.
- Author:
Dong Gu JEONG
1
;
Eui Suk JEONG
;
Jin Hee SEO
;
Seung Ho HEO
;
Yang Kyu CHOI
Author Information
- Publication Type:Original Article
- Keywords: Genetic background; knockout mouse; Streptococcus pneumoniae
- MeSH: Animals; Bacterial Load; Body Weight Changes; Interleukin-10; Mice; Mice, Knockout; Pneumococcal Infections; Pneumonia; Sepsis; Streptococcus; Streptococcus pneumoniae; Survival Rate; Tumor Necrosis Factor-alpha
- From:Laboratory Animal Research 2011;27(2):91-98
- CountryRepublic of Korea
- Language:English
- Abstract: Streptococcus pneumoniae is a major pathogen that causes various diseases, including pneumonia and sepsis, as millions of people suffer from S. pneumoniae infection worldwide. To better understand the immune and inflammatory responses to S. pneumoniae, we produced murine models. To investigate the differences between intranasal and intratracheal infection, BALB/c mice were infected with S. pneumoniae D39 intranasally or intratracheally. Mice showed no significant differences in survival rates, body weight changes, and bacterial loads. To investigate resistance and susceptibility among mouse strains, BALB/c, C57BL/6J, tumor necrosis factor-alpha (TNF-alpha) knockout, and interleukin-10 (IL-10) knockout mice were infected with S. pneumoniae D39 via intranasal or intravenous routes. In this study, BALB/c and C57BL/6J mice were resistant, IL-10 knockout mice were intermediate, and TNF-alpha knokout mice were susceptible to S. pneumoniae infection. These data show that intranasal and intratracheal infection induced similar results after S. pneumoniae infection, and the genetic background of mice must be considered when studying S. pneumoniae infection in vivo.
