Study on the characteristic chromatogram of Shexiang Jiegu Capsule and determination of seven components by HPLC
10.12206/j.issn.2097-2024.202307059
- VernacularTitle:麝香接骨胶囊的HPLC特征图谱的研究及7种成分含量测定
- Author:
Xiaocui YU
1
;
Xiwen WANG
1
;
Guiying ZHANG
1
;
Junwei XU
1
;
Yuwei ZHU
1
;
Dan HU
1
Author Information
1. Administration for Drug and Instrument Supervision and Inspection of PLAJLSF, Beijing 100166, China.
- Publication Type:Originalarticles
- Keywords:
Shexiang Jiegu Capsules;
characteristic chromatogram;
HPLC;
quality control
- From:
Journal of Pharmaceutical Practice and Service
2025;43(8):395-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the characteristic atlas of Shexiang Jiegu Capsule and determine the contents of seven active components （hydroxysafflor Yellow A, paeoniflorin, ferulic acid, naringin, ligustilide, catechin, epicatechin）. Methods Octadecyl silane bonded silica gel was used as the filling agent, the mobile phase was composed of methanol-0.05% phosphoric acid by gradient elution, the detection wavelength was 245 nm, flow rate was 1.0 ml/min, column temperature was 30℃. The similarity of the fifteen batches of sample was evaluated in line with the TCM Chromatographic Fingerprint （2012 edition）, and the contents of seven active components were determined. Results The HPLC fingerprint of Shexiang Jiegu Capsules was established. The similarity of fingerprint between fifteen batches of samples and control fingerprint was between 0.893 and 0.992. The results of methodological investigation for the determination of seven active components in fifteen batches of samples all met the requirements. Conclusion The established characteristic atlas of Shexiang Jiegu Capsules had high specificity and good repeatability, which could provide scientific basis for quality control of Shexiang Jiegu Capsules.
