Analysis of chemical components，prototype components migrating to the blood and metabolites of Sanhua decoction
- VernacularTitle:三化汤的化学成分、入血原型成分及代谢成分分析
- Author:
Yanping LIU
1
;
Ye CHEN
1
;
Tao LU
1
;
Juanmin TAO
1
;
Yan QIU
1
Author Information
1. Dept. of Pharmacy，Shanghai Pudong New Area People’s Hospital，Shanghai 201299，China
- Publication Type:Journal Article
- Keywords:
Sanhua decoction;
chemical components;
prototype components migrating to the blood;
metabolites;
UHPLC-Q-
- From:
China Pharmacy
2025;36(14):1760-1764
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the chemical components， components migrating to the blood and metabolites of Sanhua decoction in rats. METHODS Male Wistar rats were divided into administration group and blank group， with 6 rats in each group. The rats in the administration group were given 13.3 g/kg of Sanhua decoction lyophilized powder solution by gavage once a day in the morning and evening， and the rats in the blank group were given an equal volume of saline by gavage once a day in the morning and evening， both for 3 consecutive days. Plasma samples were collected from the two groups of rats after the last administration. The chemical components， prototype components migrating to the blood and metabolites of Sanhua decoction were analyzed by UHPLC-Q-TOF/MS technique. The structures were identified combined with the self-built natural product high- resolution mass spectrometry database and relevant literature. RESULTS & CONCLUSIONS Totally 69 compounds were identified from the lyophilized powder of Sanhua decoction， including 29 components from Rheum officinale， 16 components from Magnoliae Officinalis Cortex， 22 components from Aurantii Fructus Immaturus， and 10 components from Notopterygii Rhizoma et Radix. Among them， 3 components （citric acid， L-tyrosine， adenosine） were present in all 4 medicinal herbs. Another component （feruloylgluconic acid） still needed to be specifically attributed. A total of 43 prototype components migrating to the blood were identified， including flavonoids， phenols， anthraquinones， phenylpropanoids， alkaloids and triterpenoids. A total of 61 metabolites were identified， predominantly consisting of flavonoids， anthraquinones and phenylpropanoids. The metabolic pathways mainly involved phase Ⅰ metabolic reactions such as demethylation and phase Ⅱ metabolic reactions like sulfation and glucuronidation.
