Circular RNA hsa_circ_0081621 promotes malignant biological behavior of human laryngeal squamous cell carcinoma cells
10.3872/j.issn.1007-385x.2025.06.003
- VernacularTitle:环状RNA hsa_circ_0081621促进人喉鳞状细胞癌细胞的恶性生物学行为
- Author:
Shenghui LIU
1
;
Xueying AI
;
Yuru XU
;
Shuqi LI
;
Meng LIU
;
Guobin HU
;
Baoen SHAN
Author Information
1. 河北医科大学第四医院 耳鼻喉科,河北 石家庄 050011;河北医科大学第四医院 科研中心,河北 石家庄 050011
- Publication Type:Journal Article
- Keywords:
laryngeal squamous cell carcinoma(LSCC);
AMC-HN-8 cell;
TU177 cell;
circular RNA;
hsa_circ_0081621;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2025;32(6):579-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of circular RNA(circRNA)hsa_circ_0081621 on the malignant biological behaviors of human laryngeal squamous cell carcinoma AMC-HN-8 and TU177 cells.Methods:AMC-HN-8 and TU177 cells were routinely cultured.si-NC,si-hsa_circ_0081621,empty vector(vector),and hsa_circ_0081621 overexpression vector(hsa_circ_0081621-OE)were transfected into AMC-HN-8 and TU177 cells,namely si-NC,si-hsa_circ_0081621,vector,and hsa_circ_0081621-OE groups,respectively.The effects of knockdown or overexpression of hsa_circ_0081621 on the proliferation,migration,and invasion of AMC-HN-8 and TU177 cells were detected by CCK-8 assay,colony formation assay,scratch wound healing assay,and Transwell chamber assay.Results:Successful knockdown or overexpression of hsa_circ_0081621 was achieved in AMC-HN-8 and TU177 cells.hsa_circ_0081621 knockdown significantly inhibited while hsa_circ_0081621 overexpression significantly promoted the proliferation,migration,and invasion of AMC-HN-8 and TU177 cells(P<0.01 or P<0.001 or P<0.0001).Conclusion:hsa_circ_0081621 promotes the malignant biological behaviors of human laryngeal squamous cell carcinoma AMC-HN-8 and TU177 cells.
