Enhancement of Microglial Phagocytosis by Scaffold Protein PDLIM5 and Its Role in Multiple Sclerosis
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2025.0201
- VernacularTitle:支架蛋白PDLIM5增强小胶质细胞吞噬及其在多发性硬化中作用的研究
- Author:
Hailian CHEN
1
;
Yuge WANG
;
Yu CUI
;
Suning PING
;
Yuan CHEN
Author Information
1. 中山大学医学院,广东 深圳 518107
- Publication Type:Journal Article
- Keywords:
PDLIM5;
multiple sclerosis;
microglia;
phagocytosis;
neuroinflammation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(2):179-185
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the expression of scaffold protein PDLIM5 in multiple sclerosis(MS)patients and the mouse microglial cell line BV2,and to explore its effects on the phagocytosis of microglial cells.[Methods]Peripheral blood samples were collected from 24 MS patients and 6 healthy volunteers as controls.The expression levels of PDLIM5 were detected by real-time quantitative PCR.A neuroinflammation cell model was established by treating the mouse microglial cell line BV2 with lipopolysaccharide(LPS,1 μg/mL).The expression levels of PDLIM5 were measured by Western Blot.The effect of PDLIM5 expression on phagocytosis was analyzed by transfecting BV2 cells with PDLIM5 shRNA plasmids or PDLIM5 overexpression plasmids.[Results]Real-time quantitative PCR results showed that compared with the healthy control group,the expression level of PDLIM5 from the MS patients was significantly increased in monocytes[2.78(0.70-6.86)vs.0.54(0.39-1.51),P=0.036]and lymphocytes[1.62(0.90-2.26)vs.0.11(0.05-0.21),P<0.001].Western Blot results indicated that PDLIM5 expression was significantly upregulated in BV2 cells following LPS stimulation(P<0.05).Plasmid transfection experiments demonstrated that knockdown of PDLIM5 inhibited the phagocytic capacity of BV2 cells as measured by trypan blue uptake(P<0.05),while overexpression of PDLIM5 enhanced the phagocytic ability of BV2 cells(P<0.001).[Conclusion]Under neuroinflammatory conditions,PDLIM5 expression is elevated,and this upregulation promotes the phagocytosis of microglial cell.
