Performance comparison of domestic and imported CD3/CD28 activation beads for CAR-T cell manufacturing
10.3872/j.issn.1007-385x.2025.05.006
- VernacularTitle:国产与进口CD3/CD28激活磁珠制备CAR-T细胞的性能对比
- Author:
Xianzheng WEI
1
;
Shuyu ZHANG
;
Yuhang JIN
;
Pu WANG
;
Xu WANG
;
Junnian ZHENG
;
Dan LIU
;
Ming SHI
Author Information
1. 徐州医科大学肿瘤研究所,江苏 徐州 221004;徐州医科大学附属医院临床肿瘤中心,江苏 徐州 221004;江苏省肿瘤生物治疗协同创新中心,江苏 徐州 221004
- Publication Type:Journal Article
- Keywords:
CD3/CD28 activation beads;
CAR-T cell manufacturing;
performance comparison;
domestic alternatives
- From:
Chinese Journal of Cancer Biotherapy
2025;32(5):492-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the performance differences of domestic and imported CD3/CD28 activation beads for manufacturing CAR-T cells,providing a backup or alternative for domestic CAR-T cell research and manufacture.Methods:A mature protocol using imported CD3/CD28 activation beads with a 1∶1 ratio for CD3+T cells was implemented as research control.Domestic beads were used with gradient ratios of 1∶2,1∶1,and 2∶1 to activate T cells.72 h after T cell activation,CAR-T cells were manufactured by CAR lentiviral infection and cell proliferation was monitored at 2-,4-,and 7-days post-infection.Flow Cytometry was used to detect CAR-T cell positivity 5 days after infection and to detectCD4/CD8 phenotype of CAR-T cells and PD1+TIM3+cell exhaustion ratio 8 days after infection.Results:CAR-T cells manufactured by domestic CD3/CD28 activation beads exhibited similar phenotype compared with those manufactured by imported CD4/CD8 beads.The positive rate of CAR-T cells prepared with domestic beads was slightly lower than that of imported beads(53.7%vs 57.9%).However,the proliferation of CAR-T cells manufactured by domestic beads was about twice that of cells manufactured by imported beads,and the exhaustion level was only half that of imported beads(4.21%vs 7.91%).Moreover,the use of domestic magnetic beads was lower than that of imported magnetic beads,which was advantageous for cutting the costs of CAR-T cells research and manufacture.Conclusion:Domestic CD3/CD28 activation beads used for CAR-T cells manufacturing demonstrate comparable overall performance to their imported counterparts,showing potential as a backup or alternative for imported beads.
