Antimicrobial effect of infrared diode laser utilizing indocyanine green against Staphylococcus aureus biofilm on titanium surface
10.14368/jdras.2024.40.2.55
- Author:
Seung Gi KIM
1
;
Si-Young LEE
;
Jong-Bin LEE
;
Heung-Sik UM
;
Jae-Kwan LEE
Author Information
1. Department of Periodontology and Research Institute for Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung, Republic of Korea
- Publication Type:Original Article
- From:Journal of Dental Rehabilitation and Applied Science
2024;40(2):55-63
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpose:This study aimed to assess the antimicrobial efficacy of an 810-nm infrared diode laser with indocyanine green (ICG) against Staphylococcus aureus on sandblasted, large grit, and acid-etched (SLA) titanium surfaces, comparing its effectiveness with alternative chemical decontamination modalities.
Materials and Methods:Biofilms of S. aureus ATCC 25923 were culturedon SLA titanium disks for 48 hours. The biofilms were divided into five treatment groups: control, chlorhexidine gluconate (CHX), tetracycline (TC), ICG, and 810-nm infrared diode laser with ICG (ICG-PDT). After treatment, colony-forming units were quantified to assess surviving bacteria, and viability was confirmed through confocal laser-scanning microscope (CLSM) imaging.
Results:All treated groups exhibited a statistically significant reduction in S. aureus (P < 0.05), with notable efficacy in the CHX, TC, and ICG-PDT groups (P < 0.01). While no statistical difference was observed between TC and CHX, the ICG-PDT group demonstrated superior bacterial reduction. CLSM images revealed a higher proportion of dead bacteria stained in red within the ICG-PDT groups.
Conclusion:Within the limitations, ICG-PDT effectively reduced S. aureus biofilms on SLA titanium surfaces. Further investigations into alternative decontamination methods and the clinical impact of ICG-PDT on peri-implant diseases are warranted.
