The intervention of Jianpifuwei granule on mucosal injury of precancerous lesion of gastric cancer via the IL-6/JAK/STAT3 axis and its mechanisms
10.3872/j.issn.1007-385x.2025.04.007
- VernacularTitle:健脾复胃颗粒通过IL-6/JAK/STAT3轴干预胃癌前病变黏膜损伤及其机制
- Author:
Juying KANG
1
;
Huiying GUAN
;
Peirun LI
;
Liming ZHANG
;
Yongqiang DUAN
Author Information
1. 中国中医科学院 研究生院,北京 100700;宁夏回族医药研究所,宁夏 银川 750021;宁夏回族自治区中医医院 肝胆脾胃病科,宁夏 银川 750021
- Publication Type:Journal Article
- Keywords:
Jianpifuwei granule(JPFWG);
precancerous lesions of gastric cancer(PLGC);
IL-6/JAK/STAT3 signal pathway
- From:
Chinese Journal of Cancer Biotherapy
2025;32(4):392-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the intervention effect of Jianpifuwei granule(JPFWG)on gastric mucosal injury in rats with precancerous lesions of gastric cancer(PLGC)by regulating the IL-6/JAK/STAT3 signaling pathway and its mechanism.Methods:The PLGC model rats were established by MNNG combined with composite factors,and randomly divided into 6 groups(20 rats/group):the blank group(untreated),model group(treated with normal saline),Weimeisu group(0.05 g/mL Weimeisu),JPFWG low-dose group(JPFWG-L,0.088 g/mL),JPFWG medium-dose group(JPFWG-M,0.176 g/mL)and JPFWG high-dose group(JPFWG-H,0.351 g/mL).After being treated with Weimeisu and different doses of JPFWG for 12 weeks,the animals were anesthetized and sacrificed,and the gastric tissues were collected.The pathological changes of gastric mucosa were observed by H-E staining,and the expression levels of IL-6-mediated JAK/STAT3 signaling pathway factors(including IL-6,JAK and STAT3)and the mRNA and proteins of their downstream target genes c-Myc and cyclin D1 in gastric mucosal tissues were detected by immunohistochemistry,qPCR and WB.Results:Compared with that in the model group,the gastric mucosal inflammatory cell infiltration was reduced in the Weimeisu group and the low,medium,and high dose groups of JPFWG;pathological condition was improved,most obviously in the high dose group of JPFWG;The protein expressions of IL-6,JAK1 and STAT3 decreased significantly(P<0.05 or P<0.01).The expressions of mRNA and proteins of c-Myc and cyclin D1 in the gastric mucosal tissues of the rats in the Weimeisu group and JPFWG-H group decreased significantly(P<0.05 or P<0.01).Conclusion:JPFWG can improve the histopathological changes of gastric mucosa in rats with PLGC,and its mechanism may be down-regulating the expressions of c-Myc and cyclin D1 through regulating IL-6/JAK/STAT3 signal pathway,thus blocking inflammation-cancer transformation process.
